Background

To examine in vitro acoustic property of nonalcoholic fatty disease in mouse and human liver to identify nonalcoholic steatohepatitis (NASH).

Methods

The acoustic impedance (× 10⁶ kg/m²/s) was measured in 35 free fatty acids (FFAs, 500 mmol/L) and histologically-diagnosed liver samples of twelve mice (four control, four simple steatosis [SS], and four NASH) and eight humans (two control, three SS, and three NASH), using 80-MHz acoustic microscopy. The sum of percentage (SP) composition of FFAs (SP-FFAs) was also assessed.

Results

Median impedance of all FFAs was 0.7 (5 FFAs with impedance 0.7); 17 FFAs with impedance < 0.7 were classified as low-impedance group; and, 13 FFAs with impedance > 0.7 were classified as high-impedance group. The median impedance of the mouse liver decreased from control (1.715), to SS (1.68), to NASH (1.635) (control versus NASH, p = 0.039 without significant differences for the other comparisons, p ≥ 0.1). Similarly, the median impedance of human liver showed decreased from control (1.825), to SS (1.788), to NASH (1.76) (control versus SS, p = 0.023; control versus NASH, p = 0.003; SS versus NASH, p = 0.050). The ratio of SP-FFAs between the low-impedance and high-impedance groups showed an increase in both mice and humans, with significant differences in mice (control versus SS, p < 0.001; control versus NASH, p < 0.001; SS versus NASH, p = 0.003), without significant differences in humans (p ≥ 0.671).

Conclusion

Lower acoustic impedance based on the intrahepatic composition of FFAs may be characteristic of NASH.

中文翻译：

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基于游离脂肪酸的低阻抗肝图像：非酒精性脂肪性肝炎（NASH）的特征性表现。

背景

要检查小鼠和人类肝脏中非酒精性脂肪病的体外声学特性，以鉴定非酒精性脂肪性肝炎（NASH）。

方法

在十二种小鼠的35种游离脂肪酸（FFA，500 mmol / L）和经组织学诊断的肝脏样本中测量了声阻抗（×10 ⁶ kg / m ² / s）（四只对照组，四只简单脂肪变性[SS]和4个NASH）和8个人类（两个对照组，三个SS和三个NASH），使用80 MHz声学显微镜。还评估了FFA（SP-FFA）的百分比（SP）组成总和。

结果

所有FFA的中值阻抗为0.7（5个FFA的阻抗为0.7）；17个阻抗<0.7的FFA被归为低阻抗组；将13个阻抗> 0.7的FFA归为高阻抗组。小鼠肝脏的平均阻抗从控制（1.715）降低，到SS（1.68），对NASH（1.635）（控制与NASH，p = 0.039，而不对其他比较显著差异，p ≥0.1）。同样，人肝的中位阻抗显示从对照组（1.825）降低到SS（1.788），再到NASH（1.76）（对照组与SSH，p = 0.023；对照组与NASH，p = 0.003； SS与NASH，p= 0.050）。低阻抗组和高阻抗组之间的SP-FFA比例在小鼠和人类中均表现出增加，但在小鼠中有显着差异（对照组与SS，p <0.001；对照组与NASH，p <0.001； SS与NASH ，p = 0.003），而不会在人体中（显著差异p）≥0.671。

结论

基于FFA肝内成分的较低声阻抗可能是NASH的特征。