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Intrinsic Post-Ejaculation Sperm Ageing Does Not Affect Offspring Fitness in Atlantic salmon
Journal of Evolutionary Biology ( IF 2.1 ) Pub Date : 2020-02-07 , DOI: 10.1111/jeb.13590
Cosima Hotzy 1 , Bao Xuhui 1 , Tuuli Larva 1 , Simone Immler 2
Affiliation  

Post‐meiotic sperm ageing, both before ejaculation and after ejaculation, has been shown to negatively affect offspring fitness by lowering the rate of embryonic development, reducing embryonic viability and decreasing offspring condition. These negative effects are thought to be caused by intrinsic factors such as oxidative stress and ATP depletion or extrinsic factors such as temperature and osmosis. Effects of post‐ejaculation sperm ageing on offspring fitness have so far almost exclusively been tested in internal fertilizers. Here, we tested whether intrinsic post‐ejaculation sperm ageing affects offspring performance in an external fertilizer, the Atlantic salmon Salmo salar. We performed in vitro fertilizations with a split‐clutch design where sperm were subjected to four post‐ejaculation ageing treatments. We varied the duration between sperm activation and fertilization while minimizing extrinsic stress factors and tested how this affected offspring fitness. We found no evidence for an effect of our treatments on embryo survival, hatching time, larval standard length, early larval survival or larval growth rate, indicating that intrinsic post‐ejaculation sperm ageing may not occur in Atlantic salmon. One reason may be the short life span of salmon sperm after ejaculation. Whether our findings are true in other external fertilizers with extended sperm activity remains to be tested.

中文翻译:

内在射精后精子老化不影响大西洋鲑鱼的后代健康

减数分裂后精子老化,无论是在射精前还是射精后,都已被证明会降低胚胎发育速度、降低胚胎活力和降低后代状况,从而对后代健康产生负面影响。这些负面影响被认为是由氧化应激和 ATP 消耗等内在因素或温度和渗透等外在因素引起的。到目前为止,射精后精子老化对后代健康的影响几乎只在内部肥料中进行了测试。在这里,我们测试了内在的射精后精子老化是否会影响外部肥料大西洋鲑鱼鲑鱼的后代表现。我们采用分体式离合器设计进行体外受精,其中精子经过四次射精后老化处理。我们改变了精子激活和受精之间的持续时间,同时最大限度地减少了外在压力因素,并测试了这如何影响后代健康。我们没有发现我们的治疗对胚胎存活、孵化时间、幼虫标准长度、早期幼虫存活或幼虫生长率有影响的证据,表明大西洋鲑鱼可能不会发生射精后精子内在衰老。原因之一可能是射精后鲑鱼精子的寿命很短。我们的研究结果是否适用于其他具有延长精子活性的外部肥料仍有待测试。早期幼虫存活或幼虫生长率,表明大西洋鲑鱼可能不会发生内在的射精后精子老化。原因之一可能是射精后鲑鱼精子的寿命很短。我们的研究结果是否适用于其他具有延长精子活性的外部肥料还有待测试。早期幼虫存活或幼虫生长率,表明大西洋鲑鱼可能不会发生内在的射精后精子老化。原因之一可能是射精后鲑鱼精子的寿命很短。我们的研究结果是否适用于其他具有延长精子活性的外部肥料还有待测试。
更新日期:2020-02-07
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