Some species of Salix sp. (willows) are a potential phytoremediator that can accumulate substantial contents of mineral elements and, therefore, to detoxify soils contaminated with pollutants and heavy metals such as the zinc (Zn). However, high concentrations of Zn inhibit plant growth and reduce biomass production in plants. In an attempt to overcome this inconvenience and to enhance plant tolerance to Zn toxicity, we tested a new tolerance induction approach by acclimation in two clones of Salix pedicellata, named SPK-12 and SP-K20. The approach comprises two successive phases. The first is a "tolerance induction phase" consisting of gradual exposure of plants to low concentrations of Zn sulfate (ZnSO4) at regular intervals until reaching DI100 (ZnSO4 inhibitory concentration). And, the second is a "tolerance maintenance phase" to uphold the acquired tolerance to Zn toxicity. The SP-K20 clone was acclimated to DI100 threshold over 33 days without noticeable symptoms of chlorosis or growth inhibition. Compared to controls, the SP-K20 clone was able to accumulate high concentrations of Zn, suggesting that phytoremediation abilities of S. pedicellata have been improved throughout the applied approach. Acclimated Salix plants might thus improve metal phytoextraction in heavily polluted soils without biomass growth inhibition.

中文翻译：

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采用新的驯化方法改善小叶柳的锌的植物修复特性。

柳属的一些种。（柳树）是一种潜在的植物修复剂，可以积累大量矿物质元素，因此可以使被污染物和重金属（例如锌（Zn））污染的土壤排毒。但是，高浓度的锌会抑制植物的生长并降低植物的生物量产生。为了克服这种不便并增强植物对锌毒性的耐受性，我们通过驯化了两个Salix pedicellata克隆，分别命名为SPK-12和SP-K20，测试了一种新的诱导诱导方法。该方法包括两个连续的阶段。第一个是“耐受诱导期”，包括以固定间隔将植物逐渐暴露于低浓度的硫酸锌（ZnSO4），直到达到DI100（ZnSO4抑制浓度）。而且，第二个是“ 整个应用方法中的花梗都得到了改善。因此，驯化的柳柳植物可能会改善重度污染土壤中的金属植物提取，而不会抑制生物量的生长。在整个应用方法中，花梗都得到了改善。因此，驯化的柳柳植物可能会改善重度污染土壤中的金属植物提取，而不会抑制生物量的生长。