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Silver nanoparticle from whole cells of the fungi Trichoderma spp. isolated from Brazilian Amazon
Biotechnology Letters ( IF 2.7 ) Pub Date : 2020-02-05 , DOI: 10.1007/s10529-020-02819-y
Matheus M Ramos 1 , Edmilson Dos S Morais 1 , Iracirema da S Sena 1 , Adilson L Lima 2 , Fábio R de Oliveira 3 , Calleu M de Freitas 3 , Caio P Fernandes 4 , José Carlos T de Carvalho 5 , Irlon M Ferreira 1
Affiliation  

Metal nanoparticles are a promising approach for the development of new antimicrobial systems. Silver nanoparticles (AgNP) have a significant antibacterial activity through bacterial surface adsorption and oxidative stress induction, as indicated by recent observations. This research aimed to use endophytic fungi from the genus Trichoderma spp. isolated from the Bertholletia excelsa (Brazil-nut) seeds and the soil to biosynthesize AgNPs and also test their antibacterial activity. The use of these fungi for this purpose not only valorizes the Amazon biodiversity but it also uses cleaner and cheaper processes, being part of the Green Chemistry concept. The particles were analyzed through Ultraviolet–Visible Spectroscopy and ZetaSizer and the band of absorption at 420 nm was analyzed through Localized Surface Plasmon Resonance. After characterization, the AgNP were tested for antibacterial activity against several bacterial strains, when it was observed that their antibacterial activity was superior in Gram-negative bacteria.

中文翻译:

来自真菌木霉属全细胞的银纳米颗粒。与巴西亚马逊隔离

金属纳米颗粒是开发新型抗菌系统的一种很有前景的方法。最近的观察表明,银纳米粒子 (AgNP) 通过细菌表面吸附和氧化应激诱导具有显着的抗菌活性。本研究旨在使用木霉属的内生真菌。从 Bertholletia excelsa(巴西坚果)种子和土壤中分离,以生物合成 AgNPs 并测试其抗菌活性。为此目的使用这些真菌不仅可以使亚马逊的生物多样性增值,而且还使用更清洁、更便宜的工艺,这是绿色化学概念的一部分。通过紫外-可见光谱和 ZetaSizer 分析颗粒,并通过局部表面等离子体共振分析 420 nm 处的吸收带。定性后,
更新日期:2020-02-05
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