Given that the pesticide and fungicide residues determination in honey is not a routine analysis in Romania, information on these emerging contaminants is useful for consumer's safety. High resolution mass spectrometry technique was applied by Q-Exactive Orbitrap LC-MS/MS to identify and quantify environmental contaminants in honey. A list of 25 compounds, biocides and antifungals was selected for the method development, based on the occurrence in the Romanian environment and their potential usage in agriculture. The method was applied for 18 various honey samples collected in different geographic regions of Romania. Eleven compounds were present in the honey samples: carbendazim, enilconazole, hexaconazole, penconazole, tebuconazole, flusilazole, thiabendazole, terconazole, cyproconazole, propiconazole, metalaxyl. Targeted MS/MS analyses were performed for confirmation. The measured quantities ranged from 1.7-7.2 μg kg-1, lower than MRLs established by the legislation. The most abundant compound was enilconazole (imazalil), which was detected in fourteen samples. To the best of our knowledge, the present study is the first one concerning antifungal contamination of honey in Romania. The results proved that the tested honey samples are safe for human consumption.

中文翻译：

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高分辨率质谱法分析罗马尼亚各种蜂蜜样品中的抗真菌残留物。

鉴于在罗马尼亚，蜂蜜中农药和杀真菌剂残留的测定不是常规分析，因此有关这些新兴污染物的信息对于消费者的安全很有用。Q-Exactive Orbitrap LC-MS / MS应用高分辨率质谱技术鉴定和定量蜂蜜中的环境污染物。根据在罗马尼亚环境中的存在及其在农业中的潜在用途，选择了25种化合物，杀生物剂和抗真菌剂进行方法开发。该方法应用于罗马尼亚不同地理区域收集的18种蜂蜜样品。蜂蜜样品中存在11种化合物：多菌灵，依尼康唑，己康唑，戊康唑，戊唑醇，氟硅唑，噻菌灵，叔康唑，环丙康唑，丙康唑，甲霜灵。进行有针对性的MS / MS分析以确认。测得的量为1.7-7.2μgkg-1，低于法律规定的最大残留限量。含量最高的化合物是依尼康唑（依玛西利），在十四个样品中检测到。据我们所知，本研究是罗马尼亚第一个关于蜂蜜的抗真菌污染的研究。结果证明测试的蜂蜜样品可以安全食用。