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Functionality and DNA-damage properties of blood cells in lactating cows exposed to glyphosate contaminated feed at different feed energy levels
Archives of Animal Nutrition ( IF 2 ) Pub Date : 2020-02-05 , DOI: 10.1080/1745039x.2020.1718474
Karina Schnabel 1 , Rolf Schmitz 2 , Jana Frahm 1 , Ulrich Meyer 1 , Gerhard Breves 2 , Sven Dänicke 1
Affiliation  

ABSTRACT Glyphosate (GL) inhibits the aromatic amino acid biosynthesis in plants and is worldwide the most used non-selective herbicide. Less is known about in vivo effects of GL contaminated feedstuffs on the health of dairy cows. The aim of this study was to examine the effects of GL residues in feed at different concentrate feed proportions (CFP) on haematology, immunological and redox parameters and on DNA-damage of blood cells in lactating dairy cows. During a 16-trial, 61 German Holstein cows (207 ± 49 d in milk; mean ± SD) were fed the same ration in week 0. Afterwards, they were assigned to either a group receiving a GL contaminated or a group receiving an uncontaminated total mixed ration (CON). Each group was subdivided into a “low concentrate” group (LC) and a “high concentrate” group (HC) with an energy content of 6.63 MJ NEL and 7.18 MJ NEL/kg dry matter, respectively. The diets were offered for ad libitum consumption. Blood samples were taken at weeks 0, 4, 8, 12 and 16. All blood samples were analysed for white and red blood cell counts. T-cell subpopulations, oxidative burst capability of leukocytes, apoptosis rate, phagocytic activity, activities of superoxide dismutase and glutathione peroxidase, the total non-enzymatic antioxidative capacity, viability and stimulation capacity of peripheral blood mononuclear cells and micronucleus- and comet assay on bovine leukocytes were measured only in week 16. The average individual GL intake of groups CON, GLLC and GLHC was 1.2 µg, 112.6 µg and 132.8 µg per kg body weight and day, respectively. GL contamination did not affect any of the tested parameters whereas CFP and time-influenced leukocytes, granulocytes, red blood cells, haemoglobin, haematocrit, mean corpuscular volume, mean corpuscular haemoglobin and CD4+ T-cells in an interactive manner characterised by a time-dependent increase in HC groups. It can be concluded that GL and GL in combination with different CFP showed no influence on any of the tested endpoints, whereas CFP and time influenced most parameters in an interactive manner.

中文翻译:

暴露于不同饲料能量水平的草甘膦污染饲料的泌乳奶牛血细胞的功能和 DNA 损伤特性

摘要 草甘膦 (GL) 可抑制植物中芳香族氨基酸的生物合成,是世界上使用最广泛的非选择性除草剂。GL 污染的饲料对奶牛健康的体内影响知之甚少。本研究的目的是检查饲料中不同浓缩饲料比例 (CFP) 的 GL 残留对泌乳奶牛血液学、免疫学和氧化还原参数以及血细胞 DNA 损伤的影响。在 16 次试验中,61 头德国荷斯坦奶牛(牛奶 207 ± 49 天;平均值 ± SD)在第 0 周喂食相同的日粮。之后,它们被分配到接受 GL 污染的组或接受未受污染的组总混合日粮 (CON)。每组又细分为“低浓度”组 (LC) 和“高浓度”组 (HC),能量含量为 6.63 MJ NEL 和 7。分别为 18 MJ NEL/kg 干物质。提供饮食以供随意食用。在第 0、4、8、12 和 16 周采集血样。分析所有血样的白细胞和红细胞计数。T 细胞亚群、白细胞的氧化爆发能力、细胞凋亡率、吞噬活性、超氧化物歧化酶和谷胱甘肽过氧化物酶的活性、总非酶抗氧化能力、外周血单核细胞的活力和刺激能力以及微核和彗星试验对牛仅在第 16 周测量白细胞。CON、GLLC 和 GLHC 组的平均个体 GL 摄入量分别为每公斤体重和每天 1.2 µg、112.6 µg 和 132.8 µg。GL 污染不影响任何测试参数,而 CFP 和受时间影响的白细胞、粒细胞、红细胞、血红蛋白、血细胞比容、平均红细胞体积、平均红细胞血红蛋白和 CD4+ T 细胞以交互方式显示,特征在于 HC 组的时间依赖性增加。可以得出结论,GL 和 GL 与不同 CFP 的组合对任何测试端点均没有影响,而 CFP 和时间以交互方式影响大多数参数。
更新日期:2020-02-05
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