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Investigating genetic variability in Hsp70 gene-5'-fragment and its association with thermotolerance in Murrah buffalo (Bubalus bubalis) under sub-tropical climate of India.
Cell Stress and Chaperones ( IF 3.8 ) Pub Date : 2020-02-04 , DOI: 10.1007/s12192-020-01075-3
Birendra Kumar 1 , Ajit Kumar Sahoo 1 , Shanker Dayal 2 , Ananta Kumar Das 1 , Subhash Taraphder 1 , Subhasis Batabyal 3 , Pradeep Kumar Ray 2 , Rajni Kumari 2
Affiliation  

The present study was undertaken to investigate genetic variability in a fragment comprising 5′UTR along with partial coding sequence of Hsp70 gene and its association with thermotolerance traits in Murrah buffalo at ICAR-Research Complex for Eastern Region, Patna (India). The allelic variants were identified from genomic DNA samples using SSCP technique. The PCR products were sequenced and analyzed. Data on different thermotolerance traits recorded in three seasons were analyzed by least squares ANOVA taking the SSCP genotypes as fixed effect. Two allelic variants (A and B), each of 503-bp in size, were documented with frequency of 0.59 and 0.41, respectively, and three genotypes (AA, AB and BB) with corresponding frequency of 0.30, 0.58 and 0.12. The allelic variants were due to single nucleotide substitution at 55th base position leading to a change of threonine (A) to methionine (B) in amino acid sequence. Both the allelic variants had 99.8% similarity in nucleotide sequence. In phylogenetic tree, allele A was in a cluster while allele B and Gangatiri cattle sequence formed a different cluster. The SSCP genotypes had significant effect on different thermotolerance traits in summer with thermo-humidity index of ≥ 84. Buffaloes with AA genotype had the highest (P ˂ 0.05) summer evening rectal temperature, respiration rate and pulse rate, inferring that the buffaloes carrying AA genotype had more stress in summer than those with AB and BB genotype. These SSCP genotypes might have differential role in heat shock protein response to induce thermotolerance of Murrah buffaloes in Gangetic plains.

中文翻译:

印度亚热带气候下,Hsp70基因5'片段的遗传变异性及其与耐热性的关系(Musrah buffalo(Bubalus bubalis))的调查。

本研究旨在在印度帕特纳东部地区ICAR研究中心的Murrah水牛中调查包含5'UTR和Hsp70基因的部分编码序列的片段的遗传变异性及其与耐热性状的关系。使用SSCP技术从基因组DNA样品中鉴定了等位基因变体。对PCR产物进行测序和分析。以SSCP基因型为固定效应,通过最小二乘方差分析分析三个季节记录的不同耐热性状的数据。分别记录了两个等位基因变体(A和B),每个大小为503-bp,频率分别为0.59和0.41,三个基因型(AA,AB和BB)的频率分别为0.30、0.58和0.12。等位基因变体是由于在第55个碱基位置的单核苷酸取代,导致苏氨酸(A)变为蛋氨酸(B)的氨基酸序列。两个等位基因变体在核苷酸序列上具有99.8%的相似性。在系统发育树中,等位基因A在一个簇中,而等位基因B和Gangatiri牛序列形成了一个不同的簇。在夏季,SSCP基因型对不同的耐热性状具有显着影响,其热湿指数≥84。具有AA基因型的水牛具有最高的(P  ˂0.05)夏季傍晚直肠温度,呼吸率和脉搏率,推断携带AA基因型水牛在夏季比用AB和BB基因型有更多的应力。这些SSCP基因型可能在热激蛋白反应中诱导恒河平原Murrah水牛的耐热性具有不同的作用。
更新日期:2020-02-04
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