Nitrogen (N) sources have been target in microalgae cultivation studies, considering their nutritional impact on growth and high costs. Here, we have evaluated the growth of Scenedesmus obliquus BR003, applying alternative low-cost culture media containing ammonium and urea, or combinations of both N sources. The culture media were applied for indoor and outdoor cultivation, followed by growth analyses and metabolic characterization. The alternative culture media B4 and L4 supported higher biomass production (1.4 g L-1) compared to BG11 (nitrate-based medium). In addition, the lipid percentage was higher for B4 (ammonium-based culture medium), reaching up to 25% DW. High contents of carbohydrates (60%) and proteins (40%) were also obtained in media with ammonium and urea, respectively. Considering the lower costs of alternative fertilizer-based media, using ammonium and/or urea as N sources, and the high lipid content observed, we suggest these media as viable for large-scale production of S. obliquus.

中文翻译：

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替代肥料为基础的生长培养基可支持高脂含量，而不会使斜生栅藻BR003的生长受到损害。

考虑到氮源对生长和高成本的营养影响，氮源已成为微藻培养研究的目标。在这里，我们使用了包含铵盐和尿素或两种氮源的组合的低成本低成本培养基，评估了斜生Scenedesmus BR003的生长。将培养基用于室内和室外栽培，然后进行生长分析和代谢表征。与BG11（硝酸盐基培养基）相比，替代培养基B4和L4支持更高的生物量生产（1.4 g L-1）。此外，B4（基于铵的培养基）的脂质百分比更高，达25％DW。在含铵和尿素的培养基中也分别获得了高含量的碳水化合物（60％）和蛋白质（40％）。