Avian influenza viruses (AIVs) recognize sialic acid linked α2,3 to galactose (SAα2,3Gal) glycans as receptors. In this study, the interactions between hemagglutinins (HAs) of AIVs and sulfated SAα2,3Gal glycans were analyzed to clarify the molecular basis of interspecies transmission of AIVs from ducks to chickens. It was revealed that E190V and N192D substitutions of the HA increased the recovery of viruses derived from an H6 duck virus isolate, A/duck/Hong Kong/960/1980 (H6N2), in chickens. Recombinant HAs from an H6 chicken virus, A/chicken/Tainan/V156/1999 (H6N1), bound to sulfated SAα2,3Gal glycans, whereas the HAs from an H6 duck virus did not. Binding preference of mutant HAs revealed that an E190V substitution is critical for the recognition of sulfated SAα2,3Gal glycans. These results suggest that the binding of the HA from H6 AIVs to sulfated SAα2,3Gal glycans explains a part of mechanisms of interspecies transmission of AIVs from ducks to chickens.

中文翻译：

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E190V替代H6血凝素是与硫酸盐唾液酸化聚糖受体结合并感染鸡的关键因素之一。

禽流感病毒（AIV）将与α2,3连接至半乳糖（SAα2,3Gal）聚糖的唾液酸识别为受体。在这项研究中，分析了AIV的血凝素（HA）与硫酸化的SAα2,3Gal聚糖之间的相互作用，以阐明AIV从鸭到鸡的种间传播的分子基础。揭示了HA的E190V和N192D取代提高了在鸡中从H6鸭病毒分离株A / duck / Hong Kong / 960/1980（H6N2）衍生的病毒的回收率。来自H6鸡病毒A /鸡/台南/ V156 / 1999（H6N1）的重组HA与硫酸化的SAα2,3Gal聚糖结合，而来自H6鸭病毒的HA没有。突变体HAs的结合偏好表明，E190V取代对于识别硫酸化的SAα2,3Gal聚糖至关重要。