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Antioxidant potential of biflavonoid attenuates hyperglycemia by modulating the carbohydrate metabolic enzymes in high fat diet/streptozotocin induced diabetic rats.
Redox Report ( IF 3.8 ) Pub Date : 2020-02-05 , DOI: 10.1080/13510002.2020.1722914
Sundaram Ramalingam 1, 2 , Muthu Karuppiah 3 , Muthusamy Thiruppathi 4 , Shanthi Palanivelu 5 , Sachdanandam Panchanatham 1
Affiliation  

Objectives: The present study was to isolate the biflavonoid (a bimolecular kaemferol structured molecule) and test its efficacy on oxidative stress and carbohydrate metabolic key enzymes in control and high fat diet and streptozotocin -induced diabetic rats.Methods: Type 2 diabetes was induced in male albino wistar rats by feeding them with high fat diet comprising of 84.3% standard laboratory chow, 5% lard, 10% yolk powder, cholesterol 0.2%, and 0.5% bile salt for 2 weeks. After 2 weeks, the animals were kept in an overnight fast and injected with low dose of streptozotocin (35 mg/kg, dissolved in 0.1 M sodium citrate buffer, pH 4.5).Results: At the end of the experimental period, diabetic control rats showed significant increase in plasma glucose, homeostatic model assessment of insulin resistance (HOMA-IR), glycosylated hemoglobin (HbA1c) with concomitant decrease in plasma insulin, total hemoglobin and body weight. The activities of key enzymes of carbohydrate metabolism, lipid peroxidation markers, antioxidant enzymes, glycogen content and glycogen synthase and glycogen phosphorylase were also altered in diabetic rats.Discussion: Oral administration of biflavonoid to diabetic rats significantly ameliorated all the biochemical alterations to near normal levels. The effect produced by the biflavonoid on various parameters was comparable to that of metformin.

中文翻译:

黄酮类化合物的抗氧化潜力通过调节高脂饮食/链脲佐菌素诱导的糖尿病大鼠中的碳水化合物代谢酶来减轻高血糖症。

目的:本研究旨在分离双黄酮类化合物(一种双分子卡姆酚结构分子),并测试其对对照组,高脂饮食和链脲佐菌素诱发的糖尿病大鼠氧化应激和碳水化合物代谢关键酶的功效。方法:在2型糖尿病中诱发给雄性白化病wistar大鼠喂食高脂肪饮食,持续2周,该饮食应包括84.3%的标准实验室食物,5%的猪油,10%的卵黄粉,0.2%的胆固醇和0.5%的胆盐。2周后,将动物禁食过夜,并注射低剂量的链脲佐菌素(35 mg / kg,溶于0.1 M柠檬酸钠缓冲液,pH 4.5)中。结果:实验期结束时,糖尿病对照组显示血浆葡萄糖显着增加,胰岛素抵抗的稳态模型评估(HOMA-IR),糖基化血红蛋白(HbA1c)伴有血浆胰岛素,总血红蛋白和体重的减少。糖尿病大鼠糖代谢的关键酶,脂质过氧化标志物,抗氧化酶,糖原含量,糖原合酶和糖原磷酸化酶的活性也发生了改变。讨论:对糖尿病大鼠口服黄酮类药物可明显改善所有生化改变,使其接近正常水平。双黄酮对各种参数产生的作用与二甲双胍相当。对糖尿病大鼠口服黄酮类化合物可将所有生化改变明显改善至接近正常水平。双黄酮对各种参数产生的作用与二甲双胍相当。对糖尿病大鼠口服黄酮类化合物可将所有生化改变明显改善至接近正常水平。双黄酮对各种参数产生的作用与二甲双胍相当。
更新日期:2020-12-01
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