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Serum protein analysis of nurse sharks, ginglymostoma cirratum
Journal of Aquatic Animal Health ( IF 1.2 ) Pub Date : 2020-03-11 , DOI: 10.1002/aah.10100
Leila AtallahBenson 1, 2 , Liza Merly 2, 3 , Carolyn Cray 4 , Neil Hammerschlag 1, 2
Affiliation  

Serum protein electrophoresis (EPH) is used to assess relative concentrations of blood proteins in clinical and biological studies. Serum EPH fractions have been determined for elasmobranchs using mammalian albumin, alpha 1-, alpha 2-, beta-, and gamma-globulin fractions, and have been deemed Fractions 1 through 5 respectively. However, serum EPH fraction concentration reference intervals (RI) have not been widely established for different elasmobranch species. In this study, RI for Fractions 1 through 5 were determined from 45 (27 females and 23 males) wild-caught nurse sharks Ginglymostoma cirratum in south Florida. Serum samples were isolated from whole blood following caudal venipuncture. Body condition was also measured in the field to assess the relative health of the individuals sampled. There was no relationship between body condition and serum EPH fraction concentrations. There was no difference between females and males in body condition or serum EPH fraction concentrations. Total solids and total protein values were significantly different (p < 0.001). Nurse shark serum EPH Fraction 1 was found within the mammalian albumin migrating band distance and was negligible. Fraction 2 showed no peak in the mammalian alpha 1-globulin range. A medium, thin peak in the mammalian alpha 2-globulin range represented Fraction 3. Fraction 4, in the mammalian beta-globulin range, consisted of the majority of protein observed. It was represented by a smooth, broad peak. A short, medium broad peak in the mammalian gamma-globulin range represented Fraction 5. The nurse shark serum EPH fraction RI provided in this study may be utilized to clinically evaluate the health of nurse sharks, both in captivity and in the wild, and to compare health in populations around the world experiencing various anthropogenic stressors and other environmental impacts.

中文翻译:

护士鲨,ginglymostoma cirratum 血清蛋白分析

血清蛋白电泳 (EPH) 用于评估临床和生物学研究中血液蛋白质的相对浓度。已经使用哺乳动物白蛋白、α1-、α2-、β-和 γ-球蛋白级分确定了弹鳃的血清 EPH 级分,并分别被视为级分 1 到 5。然而,尚未为不同的弹鳃物种广泛建立血清 EPH 分数浓度参考区间 (RI)。在本研究中,从佛罗里达州南部的 45 条(27 条雌性和 23 条雄性)野生护士鲨 Ginglymostoma cirratum 中确定了第 1 至 5 部分的 RI。在尾静脉穿刺后从全血中分离血清样品。还在现场测量身体状况以评估采样个体的相对健康状况。身体状况和血清 EPH 分数浓度之间没有关系。女性和男性在身体状况或血清 EPH 分数浓度方面没有差异。总固体和总蛋白质值显着不同(p < 0.001)。在哺乳动物白蛋白迁移带距离内发现了护士鲨血清 EPH 组分 1,可以忽略不计。部分 2 在哺乳动物 α 1-球蛋白范围内没有显示出峰。哺乳动物α 2-球蛋白范围内的中等薄峰代表级分3。哺乳动物β-球蛋白范围内的级分4由观察到的大部分蛋白质组成。它由一个平滑、宽阔的峰代表。哺乳动物 γ-球蛋白范围内的一个短、中宽峰代表组分 5。
更新日期:2020-03-11
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