Abstract

The present study in sheep model was to find out the interaction of apoptotic transcripts, that is, Bcl2, Bax, Casp3, PCNA and p53 and pluripotency related transcripts, that is, Sox2, Nanog and Oct4 in ovine embryos produced in vitro at different O₂ concentrations (20% and 5% O₂) to compare their developmental potential. Oxygen concentrations did not influence the maturation and cleavage rate but the percentage of morula and blastocysts was significantly more at 5% as compared to 20% O₂. A significant upregulated expression of Bcl2 and PCNA genes and significantly downregulated expression of Casp3 and p53 were observed in the blastocysts at 5% than those at 20% O₂. The expression of Bax was not influenced by the O₂ concentration. Among the pluripotency related transcripts, the expression of Oct4 was significantly upregulated and the expression of Sox2 and Nanog was significantly downregulated in embryos at 5% than at 20% O₂. The study concluded that the embryos produced in vitro at low O₂ (5%) concentration regulate the expression of developmental genes related to apoptosis and pluripotency to improve the developmental potential of embryos as compared to high O₂ (20%) concentration.

摘要

目前在绵羊模型中的研究是为了找出在不同氧条件下体外产生的绵羊胚胎中凋亡转录物（即Bcl2、Bax、Casp3、PCNA和p53）与多能性相关转录物（即Sox2、Nanog和Oct4）之间的相互作用。_{2 个}浓度（20% 和 5% O ₂）来比较它们的发展潜力。氧气浓度不影响成熟和卵裂率，但桑椹胚和胚泡的百分比在 5% 时明显高于 20% O ₂。Bcl2和PCNA 的显着上调表达在 5% 的囊胚中观察到Casp3和p53 的基因和显着下调的表达，而不是在 20% O _{2 的情况下}。Bax的表达不受O ₂浓度的影响。在多能性相关转录物中，Oct4的表达显着上调，Sox2和Nanog的表达在胚胎中显着下调，5% 比 20% O ₂。该研究得出的结论是，在低 O _{2 条件下}体外产生的胚胎(5%) 浓度调节与细胞凋亡和多能性相关的发育基因的表达，与高 O ₂ (20%) 浓度相比，可提高胚胎的发育潜力。