The chromoblastomycosis is a subcutaneous mycosis with a high morbidity rate, Fonsecaea pedrosoi being the largest etiologic agent of this mycosis, usually confined to the skin and subcutaneous tissues. Rarely people get the cure, because the therapies shown to be deficient and few studies report the host-parasite relationship. Dendritic cells (DCs) are specialized in presenting antigens to naïve T lymphocytes inducing primary immune responses. Therefore, we propose to study the migratory capacity of DCs after infection with conidia of F. pedrosoi. The phenotype of DCs was evaluated using cells obtained from footpad and lymph nodes of BALB/c mice after 12, 24 and 72 h of infection. After 24 and 72 h of infection, we found a significant decrease in DCs in footpad and a significant increase in the lymph nodes after 72 h. The expression of surface markers and co-stimulatory molecules were reduced in cells obtained from footpad. To better assess the migratory capacity of DCs migration from footpad, CFSE-stained conidia were injected subcutaneously. We found that after 12 and 72 h, CD11c+ cells were increased in regional lymph nodes, leading us to believe that DCs (CD11c+) were able to phagocytic conidia present in footpad and migrated to regional lymph nodes.

中文翻译：

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Fonsecaea pedrosoi分生孢子在实验性成色菌病过程中诱导树突状细胞激活并增加区域淋巴结中的CD11c +细胞。

色母细胞病是一种具有较高发病率的皮下真菌病，Fonsecaea pedrosoi是该真菌病的最大病因，通常局限于皮肤和皮下组织。很少有人能治愈，因为疗法显示出不足，很少有研究报道宿主与寄生虫的关系。树突状细胞（DC）专门用于将抗原呈递给幼稚的T淋巴细胞，从而诱导原发性免疫反应。因此，我们建议研究DC迁徙后的迁徙能力。在感染12、24和72小时后，使用从BALB / c小鼠的足垫和淋巴结获得的细胞评估DC的表型。感染24和72小时后，我们发现72小时后足垫DC明显减少，淋巴结明显增加。从脚垫获得的细胞中表面标志物和共刺激分子的表达降低。为了更好地评估DC从脚垫迁移的迁移能力，将CFSE染色的分生孢子皮下注射。我们发现，在12和72小时后，区域淋巴结中的CD11c +细胞增加，使我们相信DC（CD11c +）能够吞噬足垫中的分生孢子，并迁移至区域淋巴结。