Enteric bacteriophages (somatic coliphages, F-specific coliphages or both together) are now recognized as useful viral indicators in water, shellfish, and biosolids and are being progressively included in national and international sanitary regulations. Among them, somatic coliphages have an advantage in that they usually outnumber F-RNA coliphages in water environments. Their enumeration using Modified Scholten’s (MS) media, following the ISO 10705-2 standard for the growth of Escherichia coli host strain WG5, is highly efficient and a common practice worldwide. These media contain a high concentration of nutrients, which may be modified to save costs without loss of bacterial growth host efficiency. This study explored reducing the concentration of nutrients in the current formulation and/or incorporating new components to improve the host bacterial growth and/or the enumeration of somatic coliphages at an affordable analytical cost. A twofold dilution of the original MS media was found not to affect the bacterial growth rate. The addition of combinations of assayed compounds to twofold diluted MS media slightly enhanced its analytical performance without altering bacterial growth. By generating savings in both cost and time while maintaining optimal results, media dilution could be applied to design new simple applications for coliphage enumeration.

中文翻译：

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评估改性Scholten培养基中新成分的体细胞毛发生长检测。

肠噬菌体（体性噬菌体，F特异性噬菌体或两者兼而有之）现已被认为是水，贝类和生物固体中有用的病毒指示剂，并逐步纳入国家和国际卫生法规。其中，体细胞噬菌体的优势在于，在水环境中，它们通常数量超过F-RNA噬菌体。遵循ISO 10705-2大肠杆菌生长标准，使用改良的Scholten培养基（MS）进行枚举宿主菌株WG5是高效的，并且是世界范围内的惯例。这些培养基含有高浓度的营养素，可以对其进行修饰以节省成本，而不会损失细菌生长宿主的效率。这项研究探索了以可承受的分析成本降低当前配方中营养物质的浓度和/或加入新的成分以改善宿主细菌的生长和/或体细胞噬菌体计数的情况。发现原始MS培养基的两倍稀释液不会影响细菌的生长速度。将测定化合物的组合添加到两倍稀释的MS培养基中，可以稍微提高其分析性能，而不会改变细菌的生长。通过在节省成本和时间的同时保持最佳结果，