Dormant bud cryogenic preservation is a cost- and labor-efficient method of genetic resource backup compared to in vitro derived meristem shoots cryopreservation. While protocols have been developed for cryopreserving apple dormant buds, effective and reproducible protocols are yet to be developed for several temperate fruit and nut species. Dormant bud cryopreservation typically requires material to be grafted to evaluate viability and recover a plant. Forced bud development has been used on a very limited scale for cryostored dormant budwood recovery, however, it provides a labor-efficient alternative viability assessment. To increase the utility of this approach, regrowth must be optimized to allow complete plant recovery. We hypothesized that bacterial attacks are limiting regrowth, thus, an antimicrobial forcing solution can maximize regrowth potential. This study examined the effects of an antimicrobial forcing solution (8-hydroxyquinoline citrate and sucrose, 8-HQC) on the cryosurvival and recovery of dormant buds of fruit (Malus x domestica, Prunus armeniaca, Prunus avium, Prunus persica, Pyrus communis), and nut species (Juglans regia, Juglans nigra, Juglans microcarpa). Recovery and shoot development were significantly improved for all the fruit and one nut species (J. microcarpa) treated with the 8-HQC, compared to standard recovery under high humidity alone (P < 0.001). Additionally, this post cryo recovery approach led to successful in vitro shoot tip establishment across all surviving fruit species. 8-HQC embedded forced bud development method increased viability and efficiency for existing cryostored material and can be used as a benchmark to develop protocols for different crops that could potentially lead to complete plant recovery.

中文翻译：

---

抗菌强制溶液提高低温保存的温带果树休眠芽的恢复

与体外衍生的分生组织芽低温保存相比，休眠芽低温保存是一种成本和劳动力效率高的遗传资源备份方法。虽然已经制定了低温保存苹果休眠芽的协议，但尚未为几种温带水果和坚果物种制定有效和可重复的协议。休眠芽冷冻保存通常需要嫁接材料以评估活力并恢复植物。强制芽开发已在非常有限的范围内用于冷冻休眠的芽材恢复，但是，它提供了一种劳动效率高的替代可行性评估。为了提高这种方法的效用，必须优化再生长以允许完全恢复植物。我们假设细菌攻击会限制再生，因此，抗菌强制解决方案可以最大限度地提高再生潜力。本研究检查了抗微生物强制溶液（8-羟基喹啉柠檬酸盐和蔗糖，8-HQC）对水果（Malus xdomestica、Prunus armeniaca、Prunus avium、Prunus persica、Pyrus community）的冷冻存活和恢复的影响，和坚果种类（Juglans regia、Juglans nigra、Juglans microcarpa）。与单独在高湿度下的标准恢复相比，用 8-HQC 处理的所有水果和一种坚果（J. microcarpa）的恢复和芽发育显着提高（P < 0.001）。此外，这种冷冻后恢复方法导致在所有幸存的水果物种中成功建立了体外芽尖。