当前位置: X-MOL 学术Indian J. Med. Microbiol. › 论文详情
Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)
Geographically distinct North-East Indian Helicobacter pylori strains are highly sensitive to clarithromycin but are levofloxacin resistant.
Indian Journal of Medical Microbiology ( IF 1.6 ) Pub Date : 2019-01-01 , DOI: 10.4103/ijmm.ijmm_19_158
Shweta Mahant 1 , Amresh Kumar Sharma 2 , Valentina Gehlot 1 , Asish Kumar Mukhopadhyay 3 , Ayushi Chhawchharia 1 , Sangitanjan Dutta 4 , Anil Agarwal 4 , Anup Som 2 , Kunal Das 5 , Rajashree Das 1
Affiliation  

Purpose Helicobacter pylori causes various gastro-intestinal diseases. Antibiotic resistance to commonly used antibiotics for the treatment of H. pylori infection is the major cause for treatment failure. The aim of this study is to determine the antimicrobial susceptibility pattern for clarithromycin and levofloxacin and find the evolutionary relationship of the partial sequence of 23S rRNA and gyraseA gene of H. pylori by phylogenetic analysis. Materials and Methods A total of 46 H. pylori strains were tested for clarithromycin and levofloxacin susceptibility pattern and phylogenetic tree were reconstructed by PhyML software. Results In this study, we observed that only 6.5% of North-East Indian H. pylori strains were resistant for clarithromycin showing mutation at A2143G and T2182C positions of 23S rRNA gene. Resistance for levofloxacin was observed in 89.1% of the H. pylori strains showing mutations at asparagine to lysine at 87 and aspartic acid to glycine/tyrosine/asparagine at 91 positions of gyraseA gene. The phylogenetic tree of the partial sequence of 23S rRNA and gyraseA gene depicts that the North-East Indian strains falls in different cluster when compared to other countries. Conclusions Resistance for clarithromycin was less in North-East Indian strains but high for levofloxacin indicating that first-line therapy may be best and effective for eradication of H. pylori in this region. This study is the first report that showed antibiotic susceptibility pattern for clarithromycin and levofloxacin by mutation analysis. By partial sequencing of 23s rRNA and gyraseA gene, we found that North-East Indian strains are geographically distinct.

中文翻译:

地理上独特的东北印度幽门螺杆菌菌株对克拉霉素高度敏感,但对左氧氟沙星耐药。

目的 幽门螺杆菌引起多种胃肠道疾病。对治疗幽门螺杆菌感染常用抗生素的抗生素耐药性是治疗失败的主要原因。本研究的目的是确定克拉霉素和左氧氟沙星的抗菌敏感性模式,并通过系统发育分析找出幽门螺杆菌23S rRNA和gyraseA基因部分序列的进化关系。材料和方法对总共46株幽门螺杆菌菌株进行了克拉霉素和左氧氟沙星敏感性模式检测,并通过PhyML软件重建了系统发育树。结果在这项研究中,我们观察到只有 6.5% 的东北印度幽门螺杆菌菌株对克拉霉素具有耐药性,显示 23S rRNA 基因的 A2143G 和 T2182C 位点发生突变。在 89.1% 的幽门螺杆菌菌株中观察到对左氧氟沙星的耐药性,显示 gyraseA 基因的 87 位天冬酰胺突变为赖氨酸,91 位天冬氨酸突变为甘氨酸/酪氨酸/天冬酰胺。23S rRNA 和 gyraseA 基因部分序列的系统发育树表明,与其他国家相比,东北印度菌株属于不同的簇。结论 印度东北部菌株对克拉霉素的耐药性较低,但对左氧氟沙星的耐药性较高,表明一线治疗可能是该地区根除幽门螺杆菌的最佳且有效的方法。这项研究是第一份通过突变分析显示克拉霉素和左氧氟沙星抗生素敏感性模式的报告。通过 23s rRNA 和 gyraseA 基因的部分测序,我们发现东北印度菌株在地理上是不同的。
更新日期:2020-02-04
down
wechat
bug