In this study, the different mole ratios of glucose oxidase/chitosan/dextran–aldehyde and glucose oxidase/chitosan/dextran–sulfate complexes were synthesized. The modification of glucose oxidase by non-covalent complexation with dextran and chitosan in different molar ratios was studied in order to increase the enzyme activity. The enzyme/polymer complexes obtained were investigated by UV spectrophotometer and dynamic light scattering. Activity determination of synthesized complexes and free enzyme were performed at a temperature range. The best results were obtained by C_chitosan/C_{dextran–aldehyde} = 10/1 ratio and C_chitosan/C_{dextran–sulfate} = 1/5 ratio that were used in thermal stability, shelf life, salt stress, and ethanol effect experiments. The results demonstrated that both complexes were thermally stable at 60 °C and had superior storage stability compared to the free glucose oxidase. Complexes showed higher enzymatic activity than free enzyme in the organic solvent environment using 10% ethanol. The complexes were resistant to salt stress containing 0.1 M NaCl or CaCl₂. The particle size distribution results of the triple complex evaluated the complexation of the chitosan, dextran derivative, and glucose oxidase. The average size of the triple complex in diameter was found to be 325.8 ± 9.3 nm. Overall findings suggest that the complexes of glucose oxidase, chitosan, and dextran showed significant enhancement in the enzyme activity.

在这项研究中，合成了葡萄糖氧化酶/壳聚糖/葡聚糖-醛和葡萄糖氧化酶/壳聚糖/葡聚糖-硫酸盐配合物的不同摩尔比。为了增加酶的活性，研究了通过葡聚糖和壳聚糖在不同摩尔比下的非共价复合来修饰葡萄糖氧化酶。通过紫外分光光度计和动态光散射研究获得的酶/聚合物复合物。在一定温度范围内进行合成复合物和游离酶的活性测定。最好的结果是由获得Ç_壳聚糖/ ç_葡聚糖醛= 10/1比和ç_壳聚糖/ ç_{葡聚糖硫酸}= 1/5的比率用于热稳定性，保质期，盐胁迫和乙醇效应实验。结果表明，与游离葡萄糖氧化酶相比，两种复合物在60°C时均具有热稳定性，并具有优异的储存稳定性。在使用10％乙醇的有机溶剂环境中，复合物显示出比游离酶更高的酶促活性。该配合物对含有0.1 M NaCl或CaCl _2的盐胁迫具有抗性。三重配合物的粒度分布结果评估了壳聚糖，葡聚糖衍生物和葡萄糖氧化酶的配合。发现三重络合物的平均直径直径为325.8±9.3nm。总体发现表明，葡萄糖氧化酶，壳聚糖和右旋糖酐的复合物显示出酶活性的显着增强。