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Recent advances in the diagnosis of dermatophytosis
Journal of Basic Microbiology ( IF 3.1 ) Pub Date : 2020-01-31 , DOI: 10.1002/jobm.201900675
Jubeda Begum 1 , Nasir A Mir 2 , Madhu C Lingaraju 3 , Bidyarani Buyamayum 4 , Kapil Dev 2
Affiliation  

Dermatophytosis is a disease of global significance caused by pathogenic keratinolytic fungi called dermatophytes in both animals and humans. The recent taxonomy of dermatophytes classifies them into six pathogenic genera, namely Microsporum, Trichophyton, Epidermophyton, Nannizzia, Lophophyton and Arthroderma. It is because of the delayed diagnostic nature and low accuracy of dermatophyte detection by conventional methods that paved the path for the evolution of molecular diagnostic techniques, which provide the accurate and rapid diagnosis of dermatophytosis for an appropriate, timely antifungal therapy that prevents the nonspecific over‐the‐counter self‐medication. This review focuses on the importance of rapid and accurate diagnosis of dermatophytosis, limitations of conventional methods, selection of targets in diagnosis, and factors affecting sensitivity and specificity of various molecular diagnostic technologies in the diagnosis of dermatophytosis. Generally, all the molecular techniques have a significant edge over the conventional methods of culture and microscopy in the dermatophytosis diagnosis. However, in mycology laboratory, the suitability of any molecular diagnostic technique in the diagnosis of dermatophytosis is driven by the requirement of time, economy, complexity, the range of species spectrum detected and the scale of diagnostic output required. Thus, various choices involved in the pursuit of a diagnosis of dermatophytosis are determined by the available conditions and the facilities in the laboratory.

中文翻译:

皮肤癣菌病诊断的新进展

皮肤癣菌病是一种具有全球意义的疾病,由动物和人类中称为皮肤癣菌的致病性角质溶解真菌引起。最近的皮肤癣菌分类学将它们分为六个致病属,即小孢子菌属、毛癣菌属、表皮癣菌属、Nannizzia属、Lophphyton属和节皮癣菌属。正是由于传统方法检测皮肤癣菌的延迟诊断性质和低准确度,为分子诊断技术的发展铺平了道路,分子诊断技术为皮肤癣菌病的准确和快速诊断提供了适当、及时的抗真菌治疗,防止了非特异性过度感染。 - 柜台自我药疗。本综述侧重于快速准确诊断皮肤癣菌病的重要性、常规方法的局限性、诊断目标的选择、各种分子诊断技术诊断皮肤癣菌病的敏感性和特异性的影响因素。一般来说,在皮肤癣菌病的诊断中,所有分子技术都比传统的培养和显微镜检查方法具有显着优势。然而,在真菌学实验室中,任何分子诊断技术在皮肤癣菌病诊断中的适用性是由时间、经济性、复杂性、检测到的物种谱范围和所需诊断输出规模的要求驱动的。因此,寻求皮肤癣菌病诊断所涉及的各种选择取决于实验室的可用条件和设施。在皮肤癣菌病的诊断中,所有分子技术都比传统的培养和显微镜方法具有显着优势。然而,在真菌学实验室中,任何分子诊断技术在皮肤癣菌病诊断中的适用性取决于时间、经济性、复杂性、检测到的物种谱范围和所需的诊断输出规模的要求。因此,寻求皮肤癣菌病诊断所涉及的各种选择取决于实验室的可用条件和设施。在皮肤癣菌病的诊断中,所有分子技术都比传统的培养和显微镜方法具有显着优势。然而,在真菌学实验室中,任何分子诊断技术在皮肤癣菌病诊断中的适用性是由时间、经济性、复杂性、检测到的物种谱范围和所需诊断输出规模的要求驱动的。因此,寻求皮肤癣菌病诊断所涉及的各种选择取决于实验室的可用条件和设施。复杂性、检测到的物种谱范围和所需的诊断输出规模。因此,寻求皮肤癣菌病诊断所涉及的各种选择取决于实验室的可用条件和设施。复杂性、检测到的物种谱范围和所需的诊断输出规模。因此,寻求皮肤癣菌病诊断所涉及的各种选择取决于实验室的可用条件和设施。
更新日期:2020-01-31
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