Background

Trematoda Opisthorchis felineus Rivolta, 1884 is the causative agent of dangerous parasite disease—opisthorchiasis, widespread in the Russian Federation. The details of the neuroanatomical localization of the serotoninergic and FMRFamidergic neurotransmitter elements as well as their functional roles remain not studied enough in both adult and larval forms of O. felineus. The studies in this area are important in term of the development of a new pharmacological strategy of the struggle with the causative agent of opisthorchiasis affecting the neuronal signal substances and the function of its nervous system.

Purpose

The aim of this work was the immunocytochemical study of the neurotransmitters serotonin (5-HT, 5-Hydroxitryptamine) and neuropeptide FMRFamide localization in the nervous system of the opisthorchiasis causative agent—O. felineus metacercaria. To study the relationship between the detected neurotransmitters and the muscular elements of the parasite, the muscle staining was carried out simultaneously using fluorophore-conjugated phalloidin.

Methods

The localization of 5-HTergic and FMRFamidergic nerve structures was determined by immunocytochemical method. The staining samples were analyzed using a fluorescent and confocal laser scanning microscopies.

Results

The new data on the presence and distribution of the serotonin-immunopositive (IP)- and FMRFa-IP components in the central and peripheral departments of the nervous system of O. felineus metacercaria has been obtained. Besides that a number of the new anatomical details of the nervous system organization and of the innervation of the organs and tissues in the investigated parasite have been revealed.

Conclusion

The data obtained on the presence and localization of the 5-HTergic and peptidergic (FMRFamide) components in central and peripheral departments of the nervous system of O. felineus metacercaria elaborated and expanded the existing information about the nervous system as well as the innervations of the tissues and organs in the causative agent of opistchorchiasis.

中文翻译：

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关于猫骨膜阿魏氏杆菌神经系统中5-羟色胺和FMRFamide定位的新数据。

背景

Trematoda Opisthorchis felineus Rivolta，1884年，是寄生虫病的病原体，在俄罗斯联邦广泛存在。在成年和幼年的猫O中，对5-羟色胺能和FMRF酰胺能神经递质的神经解剖学定位及其功能作用的细节尚未充分研究。该领域的研究对于开发与影响神经元信号物质及其神经系统功能的阿米巴状鞭毛虫病致病因子作斗争的新药理学策略至关重要。

目的

这项工作的目的是对神经递质5-羟色胺（5-HT，5-羟色胺）和神经肽FMRFamide定位在视神经性鞭毛病病原体猫屎。虫的神经系统中的免疫细胞化学研究。为了研究检测到的神经递质与寄生虫肌肉成分之间的关​​系，使用荧光团缀合的鬼笔环肽同时进行了肌肉染色。

方法

通过免疫细胞化学方法确定5-HT能和FMRF酰胺能神经结构的定位。使用荧光和共聚焦激光扫描显微术分析染色样品。

结果

获得了有关猫尾cer神经系统中枢和外周部5-羟色胺免疫阳性（IP）和FMRFa-IP成分的存在和分布的新数据。除此之外，还揭示了神经系统组织的许多新的解剖学细节以及所研究的寄生虫的器官和组织的神经支配。

结论

对5-HT能和在神经系统的中枢和外周肽能部门（FMRFamide）成分的存在和定位而获得的数据O. felineus囊蚴阐述和扩展有关的神经系统中的现有信息，以及的神经支配的组织器官中的阿斯匹克病的病原体。