The past six years have seen the rapid growth of studies of CRISPR/Cas9 in plant genome editing, a method that enormously facilitates both basic research and practical applications. Most studies have focused on genetic model species, but plant species that are not genetic models may also be economically important or biologically significant, or both. However, developing the CRISPR/Cas9 system in a nongenetic model is challenging. Here, we summarize CRISPR/Cas9 applications in 45 plant genera across 24 families and provide a reference for practical application of CRISPR in nongenetic model plant systems. Suggestions for selecting plant species and target genes are given for proof‐of‐principle CRISPR studies, and the processes of vector construction are reviewed. We recommend using transient assays to identify a desired CRISPR/Cas9 system in a nongenetic model. We then review methods of plant transformation and describe approaches, using regenerated transgenic plants, for evaluating CRISPR editing results. Lastly, potential future applications of CRISPR in nongenetic model plant species are discussed. This review provides a road map for developing CRISPR in nongenetic models, an application that holds enormous potential in plant biology.

中文翻译：

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在非遗传模型植物系统中适应CRISPR / Cas9的注意事项。

在过去的六年中，CRISPR / Cas9在植物基因组编辑中的研究迅速发展，这种方法极大地促进了基础研究和实际应用。大多数研究都集中在遗传模型物种上，但是非遗传模型的植物物种在经济上或生物学上也可能具有重要意义，或两者兼而有之。然而，在非遗传模型中开发CRISPR / Cas9系统具有挑战性。在这里，我们总结了CRISPR / Cas9在24个家族的45个植物属中的应用，并为CRISPR在非遗传模型植物系统中的实际应用提供了参考。提出了选择植物种类和目标基因的建议，以用于CRISPR原理验证研究，并综述了载体构建过程。我们建议使用瞬时分析在非遗传模型中鉴定所需的CRISPR / Cas9系统。然后，我们回顾植物转化的方法，并描述使用再生的转基因植物评估CRISPR编辑结果的方法。最后，讨论了CRISPR在非遗传模型植物物种中的潜在未来应用。这篇综述提供了在非遗传模型中开发CRISPR的路线图，这种应用在植物生物学中具有巨大的潜力。