Introduction: Glycomics, which aims to define the glycome of a biological system to better assess the biological attributes of the glycans, has attracted increasing interest. However, the complexity and diversity of glycans present challenging barriers to glycome definition. Technological advances are major drivers in glycomics.Areas covered: This review summarizes the main methods and emphasizes the most recent advances in mass spectrometry-based methods regarding glycomics following the general workflow in glycomic analysis.Expert opinion: Recent mass spectrometry-based technological advances have significantly lowered the barriers in glycomics. The field of glycomics is moving toward both generic and precise analysis.

中文翻译：

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糖组学新方法：2019 年更新。

简介： Glycomics 旨在定义生物系统的糖组以更好地评估聚糖的生物属性，引起了越来越多的兴趣。然而，聚糖的复杂性和多样性给糖组定义带来了挑战性的障碍。技术进步是糖组学的主要驱动力。 涵盖的领域：本综述总结了主要方法并强调了基于质谱的方法在糖组学分析一般工作流程之后的最新进展。专家意见：最近基于质谱的技术进步已经显着降低了糖组学的障碍。糖组学领域正朝着通用和精确分析的方向发展。