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Production and characterization of bacterial cellulose obtained by Gluconacetobacter xylinus utilizing the by-products from Baijiu production.
Bioprocess and Biosystems Engineering ( IF 3.8 ) Pub Date : 2020-01-29 , DOI: 10.1007/s00449-020-02289-6
Fuqiang He 1 , Huimin Yang 1 , Lilan Zeng 1 , Hong Hu 1 , Cheng Hu 1
Affiliation  

Bacterial cellulose (BC) has extensive application prospects in many fields in view of its unique characteristics. However, the large-scale applications of BC are severely limited because of relatively low BC productivity and high cost of culture medium. Herein, the distiller's grain enzymatic hydrolysate (DEH) and yellow water were successfully combined as an effective substitute (the best distiller's grains-yellow water medium, BDY medium) for traditional Hestrin-Schramm medium (HS medium) for BC production by Gluconacetobacter xylinus through the response surface methodology. The BC production in BDY medium was significantly enhanced to 7.42 g/l with BC conversion yield of 42.4% after 7 days static cultivation, which was 3.72-fold and 3.37-fold higher than that in HS medium, respectively. The structure and properties of BC membranes produced in HS and BDY medium were evaluated by X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FT-IR), field emission scanning electron microscopy (FE-SEM), thermogravimetric analysis (TGA) and hydrophilicity analysis. There was no significant difference between BC samples produced in the HS and BDY medium, indicating that BDY, as abundant and inexpensive substrates, can effectively replace HS medium to enhance BC production. The employment of distiller's grains and yellow water to BC production not only is conducive to achieve industrial production of BC, but also can effectively realize the recycling of waste from Baijiu distillery.

中文翻译:

利用来自白酒生产的副产物,由木糖葡糖杆菌获得的细菌纤维素的生产和表征。

细菌纤维素(BC)因其独特的特性而在许多领域具有广阔的应用前景。然而,由于BC生产率较低和培养基成本高,BC的大规模应用受到严重限制。在这里,酒糟的谷物酶解产物(DEH)和黄水成功地结合在一起,成为传统的Hestrin-Schramm培养基(HS培养基)的有效替代品(最佳的酒糟-黄色水培养基,BDY培养基),用于通过木糖杆菌通过响应面方法。静态培养7天后,BDY培养基中的BC产量显着提高至7.42 g / l,BC转化率为42.4%,分别比HS培养基高3.72倍和3.37倍。通过X射线衍射(XRD),傅立叶变换红外光谱(FT-IR),场发射扫描电子显微镜(FE-SEM),热重分析(TGA)对在HS和BDY介质中生产的BC膜的结构和性能进行了评估。和亲水性分析。HS和BDY培养基中产生的BC样品之间没有显着差异,表明BDY作为丰富廉价的底物可以有效替代HS培养基以提高BC产量。将酒糟和黄水用于不列颠哥伦比亚省的生产,不仅有利于实现不列颠哥伦比亚省的工业化生产,而且可以有效地实现白酒酿酒厂废物的回收利用。场发射扫描电子显微镜(FE-SEM),热重分析(TGA)和亲水性分析。HS和BDY培养基中产生的BC样品之间没有显着差异,表明BDY作为丰富廉价的底物可以有效替代HS培养基以提高BC产量。将酒糟和黄水用于不列颠哥伦比亚省的生产,不仅有利于实现不列颠哥伦比亚省的工业化生产,而且可以有效地实现白酒酿酒厂废物的回收利用。场发射扫描电子显微镜(FE-SEM),热重分析(TGA)和亲水性分析。HS和BDY培养基中产生的BC样品之间没有显着差异,表明BDY作为丰富廉价的底物可以有效替代HS培养基以提高BC产量。将酒糟和黄水用于不列颠哥伦比亚省的生产,不仅有利于实现不列颠哥伦比亚省的工业化生产,而且可以有效地实现白酒酿酒厂废物的回收利用。
更新日期:2020-04-20
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