Histoplasmosis is a worldwide systemic endemic mycosis caused by several cryptic species included within the Histoplasma capsulatum complex. Domestic and wild mammals are susceptible to infection by this fungus and could be used as indicators of its presence in the environment. The aim of the study was to identify the natural reservoirs of H. capsulatum in the Argentinean Humid Pampas eco-region analyzing a wildlife frozen-tissue collection and trace its distribution patterns over time and space. Tissue samples from 34 small wild mammals caught in the Humid Pampas were analyzed using two molecular markers: 100 kDa protein coding gene (Hcp100) and ITS1 rDNA. Results showed that 32.4% of them were infected with H. capsulatum and its DNA was detected in 5/17 Calomys laucha; 3/6 Calomys musculinus; 1/5 Akodon azarae, 1/3 Monodelphis dimidiata; and 1/2 Didelphis albiventris. In the single specimen studied of Cavia aperea, no H. capsulatum DNA was detected. This is the first H. capsulatum infection report in C. laucha and C. musculinus rodents and M. dimidiate opossum which proves that tissue collections are an important source of material for epidemiological studies of endemic disease over time.

组织胞浆菌病是一种全球性的系统性地方性真菌病，它是由包膜组织胞浆复合物中的几种隐性物种引起的。家养和野生哺乳动物易受这种真菌感染，可用作其在环境中存在的指标。该研究的目的是通过分析野生动植物冷冻组织的收集并在时间和空间上追踪其分布规律，从而确定阿根廷湿地潘帕斯生态区中荚膜荚膜梭菌的天然贮藏库。使用两个分子标记物分析了在潮湿的南美大草原中捕获的34个小型野生哺乳动物的组织样本：100 kDa蛋白质编码基因（Hcp100）和ITS1 rDNA。结果显示，其中有32.4％的患者感染了荚膜嗜血杆菌在5/17 Calomys laucha中检测到它的DNA ; 3/6鼠Calomys musculinus ; 1/5 Akodon azarae，1/3 Monodelphis dimidiata ; 和1/2 Didelphis albiventris。在研究过的Cavia aperea的单个标本中，未检测到荚膜H.荚膜DNA。这是第一个荚膜组织胞浆菌在感染报告C. laucha和C. musculinus啮齿动物和M.二分负鼠这证明组织集合是材料的随时间地方病的流行病学研究的重要来源。