The purpose of this study is to investigate the effect of soil organic matter (SOM) content levels on the biodegradation of total petroleum hydrocarbons (TPH). Batch experiments were conducted with soils with 2% or 10% organic matter that had been contaminated by diesel or fuel oil. In addition to the TPH (diesel or fuel oil) degradation efficiency, a comprehensive investigation was conducted on the TPH-degrading microbial community using molecular tools including oligonucleotide microarray technique and terminal restriction fragment length polymorphism analysis (T-RFLP). TPH was reduced from 10,000 mg/kg to 1849-4352 mg/kg dry weight soil. Higher biodegradation efficiencies and kinetic rate constants were observed in higher SOM contents. Hydrocarbon fractional analyses were conducted to explain the optimal operation with relatively low resin and aromatic fractions detected at the end of the remediation. The bacterial and fungal counts in the 10% SOM were approximately 10 CFU/g to 102 CFU/g above those in the 2% SOM, and the lowest fungal level was found when the least TPH degradability was measured. The internal transcribed spacer microarray identified the microorganisms that were introduced and proved their survival. The associated growth pattern confirmed that different kinds of contamination oils affected the microbial community diversity over time. Both the microarray and T-RFLP profiles indicated that Gordonia alkanivorans, G. desulfuricans, and Rhodococcus erythoropolis were the dominant bacteria, while Fusarium oxysporum and Aspergillus versicolor were the dominant fungi. The T-RFLP-derived nonmetric multidimensional scaling concluded that the dynamics of the microbial communities were impacted by the TPH degradation stages.

中文翻译：

---

土壤有机质对柴油/燃料油污染土壤中石油烃降解的影响。

这项研究的目的是调查土壤有机质（SOM）含量水平对总石油烃（TPH）生物降解的影响。批处理实验是在含有2％或10％有机物的土壤中被柴油或燃料油污染的。除了TPH（柴油或燃料油）的降解效率外，还使用包括寡核苷酸微阵列技术和末端限制性片段长度多态性分析（T-RFLP）在内的分子工具对TPH降解微生物群落进行了全面研究。TPH从10,000 mg / kg干重土壤减少到1849-4352 mg / kg干重土壤。在较高的SOM含量下观察到较高的生物降解效率和动力学速率常数。进行了烃馏分分析，以解释在修复结束时检测到相对较低的树脂和芳烃馏分的最佳操作。10％SOM中的细菌和真菌数量比2％SOM中的细菌和真菌数量大约高10 CFU / g至102 CFU / g，并且当测量到最低的TPH降解性时，细菌和真菌的数量最低。内部转录的间隔物微阵列鉴定出引入的微生物并证明其存活。相关的生长方式证实，随着时间的推移，不同种类的污染油会影响微生物群落的多样性。芯片和T-RFLP谱均表明，Gordonia alkanivorans，G。desulfuricans和Rhodococcus erythoropolis是主要细菌，而尖镰孢和杂色曲霉是主要真菌。