H1 histones bind to linker DNA. H1t (H1f6), a testis‐specific linker histone variant, is present in pachytene spermatocytes and spermatids. The expression of H1t histone coincides with the acquisition of metaphase I competence in pachytene spermatocytes. Here we report the generation of H1t‐GFP transgenic mice. The H1t‐GFP (H1 histone testis‐green fluorescence protein) fusion protein expression recapitulates the endogenous H1t expression pattern. This protein appears first in mid pachytene spermatocytes in stage V seminiferous tubules, persists in round spermatids and elongating spermatids, but is absent in elongated spermatids. The strong green fluorescence signal, due to the high abundance of H1t‐GFP, is maintained in spermatocytes after induction towards metaphase I through treatment with okadaic acid. Therefore, H1t‐GFP can be used as a visual marker for monitoring the progression of meiosis in vitro and in vivo, as well as fluorescence‐activated cell sorting (FACS) sorting of germ cells.

中文翻译：

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大鼠H1t-GFP转基因概括了小鼠中的内源性H1t表达模式。

H1组蛋白与接头DNA结合。H1t（H1f6）是睾丸特异性的连接蛋白组蛋白变体，存在于粗线期的精细胞和精子细胞中。H1t组蛋白的表达与粗线期精细胞中中期I能力的获得相吻合。在这里，我们报告了H1t-GFP转基因小鼠的产生。H1t-GFP（H1组蛋白睾丸绿色荧光蛋白）融合蛋白表达概括了内源性H1t表达模式。该蛋白首先出现在V期曲细精管的中期粗线精细胞中，在圆形的精子和伸长的精子中持续存在，而在细长的精子中不存在。通过冈田酸处理，诱导进入中期I后，由于H1t-GFP的丰度很高，在精母细胞中仍保持了强烈的绿色荧光信号。因此，