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Prevalence of Antimicrobial Resistance Genes and Integrons in Commensal Gram-Negative Bacteria in a College Community.
Microbial Drug Resistance ( IF 2.6 ) Pub Date : 2020-09-28 , DOI: 10.1089/mdr.2019.0279
Julia Rubin 1 , Kaitlyn Mussio 1 , Yuqi Xu 2 , Joy Suh 1 , Lee W Riley 1
Affiliation  

Although the human intestinal microbiome has been shown to harbor antimicrobial drug resistance genes (ARGs), the prevalence of such genes in a healthy population and their impact on extraintestinal infections that occur in that community are not well established. This study sought to identify ARG prevalence and their mobile elements in the intestines of a healthy community population at a California University, and compared these genes to those previously identified among uropathogenic Escherichia coli isolated from patients with urinary tract infection from the same community. We isolated Gram-negative bacteria (GNB) from fecal samples of healthy volunteers and screened them by polymerase chain reaction for class 1 integron cassette sequences and ARGs encoding resistance against ampicillin, trimethoprim-sulfamethoxazole, gentamicin, and colistin. We found antimicrobial-resistant GNB from 83 (81%) of 102 nonredundant rectal swab samples. Seventy-four (72%) of these samples contained β-lactamase genes (blaTEM, blaSHV, blaCTX-M, blaOXA, and blaOXY), dihydrofolate reductase (DHFR) genes (dhfr-A17, dhfr-A12, dhfr-A7, dhfr-A5, dhfr-A21, dhfr-A1, dhfr-A13, and dhfr-7), and aminoglycoside resistance genes (aadA5, aadA2, aadA1, and aadB). Integron sequences were found in 37 (36%) fecal samples. These genes were found in 11 different GNB species. The high prevalence of clinically common ARGs and integrons harbored by GNB in the intestine of a healthy population suggest that human intestines may serve as a major reservoir of these mobile ARGs that appear in E. coli strains causing extraintestinal infections in the same community.

中文翻译:

高校社区共生革兰氏阴性细菌中抗菌素耐药基因和整合素的流行。

尽管已证明人类肠道微生物组具有抗菌药物抗性基因(ARG),但尚不清楚此类基因在健康人群中的流行及其对在该社区中发生的肠外感染的影响。这项研究试图确定加利福尼亚大学健康社区人群肠道中的ARG流行率及其活动成分,并将这些基因与先前在尿路致病性大肠杆菌中鉴定出的基因进行比较。从同一社区的尿路感染患者中分离出来。我们从健康志愿者的粪便样本中分离出了革兰氏阴性细菌(GNB),并通过聚合酶链反应筛选了它们的1类整合子盒序列和ARGs,它们编码了对氨苄西林,甲氧苄氨磺胺甲基异恶唑,庆大霉素和大肠菌素的抗性。我们从102个非冗余直肠拭子样本中发现了83个(81%)具有抗药性的GNB。这些样品中有74(72%)个包含β-内酰胺酶基因(bla TEMbla SHVbla CTX-Mbla OXAbla OXY),二氢叶酸还原酶(DHFR)基因(DHFR-A17,DHFR-A12,DHFR-A7,DHFR-A5,DHFR-A21,DHFR-A1,DHFR-A13,DHFR-7和氨基糖苷抗性基因(aadA5,aadA2,aadA1,aadB)。在37个(36%)粪便样品中发现了整合子序列。这些基因在11种不同的GNB物种中发现。在健康人群的肠道中,GNB携带的临床常见ARG和整合素的高流行率表明,人肠道可能是这些可移动ARG的主要贮藏物,这些ARG出现在大肠杆菌菌株中,在同一社区中引起肠道外感染。
更新日期:2020-10-02
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