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Effects of zinc supplementation on metallothionein levels in ischemic renal tissue.
Biotechnic & Histochemistry ( IF 1.6 ) Pub Date : 2020-01-27 , DOI: 10.1080/10520295.2019.1691264
Betul Yazğan 1 , Abdulkerim Kasim Baltaci 2 , Rasim Mogulkoc 2 , Mustafa Cihat Avunduk 3
Affiliation  

We investigated how zinc (Zn) supplementation affects metallothionein levels in the cortex and medulla of ischemic renal tissue of rats. We used adult male rats divided into four groups: group 1, untreated control; group 2, sham-operated; group 3, ischemia-reperfusion; group 4, ischemia-reperfusion + 5 g/kg Zn. Renal tissue was analyzed using immunostaining of rat metallothionein. Cells stained with metallothionein were counted and their percentage was calculated. We found that the Zn supplemented ischemia and reperfusion group exhibited a greater percentage of cells stained strongly for metallothionein in the renal cortex than all other groups. In the renal medulla, percentages of weak staining for metallothionein in the control and ischemia and reperfusion groups were greater than those in the sham and Zn-supplemented ischemia/reperfusion groups. Our findings indicate that the main effect of Zn in the renal tissue occurs in the cortex, while metallothionein synthesis in the renal medulla is unaffected.

中文翻译:

补锌对缺血性肾组织中金属硫蛋白水平的影响。

我们调查了锌(Zn)补充剂如何影响大鼠缺血性肾组织的皮质和髓质中的金属硫蛋白水平。我们将成年雄性大鼠分为四组:第1组,未治疗的对照组;第1组,未治疗的对照组。第2组,假手术;第3组,缺血-再灌注;第4组,缺血再灌注+ 5 g / kg锌。使用大鼠金属硫蛋白免疫染色分析肾组织。计数用金属硫蛋白染色的细胞并计算其百分比。我们发现,与所有其他组相比,锌补充的缺血和再灌注组在肾皮质中的金属硫蛋白强烈染色的细胞百分比更高。在肾髓质中,对照组,局部缺血和再灌注组中金属硫蛋白的弱染色百分比高于深水组和补充锌的局部缺血/再灌注组。
更新日期:2020-01-27
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