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Evaluation of regulatory T cells frequency and FoxP3/GDF-15 gene expression in β-thalassemia major patients with and without alloantibody; correlation with serum ferritin and folate levels.
Annals of Hematology ( IF 3.5 ) Pub Date : 2020-01-27 , DOI: 10.1007/s00277-020-03931-9
Negin Shokrgozar 1 , Niloofar Amirian 1 , Reza Ranjbaran 1 , Asghar Bazrafshan 2 , Sedigheh Sharifzadeh 1
Affiliation  

β-thalassemia major is one of the most common hematologic disorders in the world. It causes severe anemia and patients require regular blood transfusions, which causes different complications such as iron overload and alloimmunization. Regulatory T cells (Tregs) have an important role in regulation of immune responses. FoxP3 is the major marker of Tregs and its expression can be influenced by different factors. GDF-15 is another gene that plays a role in iron homeostasis and regulation of immune system in different diseases. The aim of this study was to assess the frequency of Tregs and FoxP3/GDF-15 gene expression in β-thalassemia major patients with and without alloantibody as well as its correlation with different factors such as serum ferritin and folate levels. This study was conducted on 68 β-thalassemia major patients with and without alloantibodies in comparison with 20 healthy individuals with matched age and sex as control group. Enzyme-linked immunosorbent assay (ELISA), flow cytometry, and real-time PCR were performed in order to evaluate serum ferritin and folate levels, frequency of Tregs, and the expression of FoxP3 and GDF-15 genes, respectively. The percentage and absolute count of Tregs were increased in patients compared with controls (P = 0.0003), but there was no difference between responders and non-responders (P > 0.05). The Tregs count correlated positively with serum ferritin. No correlation was observed between target genes and serum ferritin and folate, but there was a positive significant correlation between the expression of FoxP3 and GDF-15 genes, which shows the immunosuppressive role of GDF-15.

中文翻译:

评估有和没有同种抗体的重型地中海贫血患者的调节性T细胞频率和FoxP3 / GDF-15基因表达;与血清铁蛋白和叶酸水平相关。

严重的β地中海贫血是世界上最常见的血液学疾病之一。它会导致严重的贫血,患者需要定期输血,这会引起不同的并发症,例如铁超负荷和同种免疫。调节性T细胞(Tregs)在调节免疫反应中起重要作用。FoxP3是Tregs的主要标志物,其表达可能受不同因素的影响。GDF-15是另一种在不同疾病中的铁稳态和免疫系统调节中起作用的基因。这项研究的目的是评估在有和没有同种抗体的β地中海贫血重症患者中Tregs和FoxP3 / GDF-15基因表达的频率,以及其与不同因素如血清铁蛋白和叶酸水平的相关性。这项研究针对68名有和没有同种抗体的重度地中海贫血患者进行了比较,而对照组则为20名年龄和性别相匹配的健康个体。为了评估血清铁蛋白和叶酸水平,Tregs的频率以及FoxP3和GDF-15基因的表达,进行了酶联免疫吸附测定(ELISA),流式细胞仪和实时PCR。与对照组相比,患者中Treg的百分比和绝对计数增加了(P = 0.0003),但有反应者和无反应者之间没有差异(P> 0.05)。Tregs计数与血清铁蛋白呈正相关。靶基因与血清铁蛋白和叶酸之间没有相关性,但FoxP3和GDF-15基因的表达呈正相关,
更新日期:2020-03-06
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