Donor cornea contamination is one of the major risks for corneal transplants. The use of antibiotics in storage media remains as one of the most important security measurements to minimize the contamination risk in corneal preservation. Since antibiotic resistance among microorganisms have been rising gradually, it is important to gain knowledge about the antimicrobial susceptibility pattern for choosing the most suitable antimicrobial agents. Thus, we evaluated the in vitro susceptibility of microorganisms isolated in donor corneas processed at the Center for Blood Transfusion, Tissues and Cells (Córdoba, Spain) during 4 years in order to evaluate the efficiency, and to promote changes for further antibiotics use. Our results show the high rate of resistance to gentamicin, an antibiotic used in corneal preservation media such as Optisol GS and Eusol-C. Conversely, all the analyzed microorganisms were sensitive to vancomycin. This suggests the possibility of replacing gentamicin with another more effective antibacterial agent such as vancomycin.

中文翻译：

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从冷藏角膜分离出的微生物的体外敏感性：增加了角膜库中庆大霉素的抗性。

供体角膜污染是角膜移植的主要风险之一。在存储介质中使用抗生素仍然是最重要的安全措施之一，可以最大程度地减少角膜保存中的污染风险。由于微生物之间的抗生素耐药性逐渐提高，因此重要的是要了解有关抗生素敏感性模式的知识，以选择最合适的抗菌剂。因此，我们评估了在4年间在输血，组织和细胞中心（西班牙科尔多瓦）加工的供体角膜中分离出的微生物的体外敏感性，以评估效率并促进抗生素进一步使用的变化。我们的结果表明对庆大霉素的耐药率很高，用于角膜保存介质（例如Optisol GS和Eusol-C）的抗生素。相反，所有分析的微生物对万古霉素敏感。这表明用另一种更有效的抗菌剂如万古霉素替代庆大霉素的可能性。