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Differential Striatal Axonal Arborizations of the Intratelencephalic and Pyramidal-Tract Neurons: Analysis of the Data in the MouseLight Database.
Frontiers in Neural Circuits ( IF 3.5 ) Pub Date : 2019-11-15 , DOI: 10.3389/fncir.2019.00071 Kenji Morita 1, 2 , Sanghun Im 3, 4 , Yasuo Kawaguchi 3, 4
Frontiers in Neural Circuits ( IF 3.5 ) Pub Date : 2019-11-15 , DOI: 10.3389/fncir.2019.00071 Kenji Morita 1, 2 , Sanghun Im 3, 4 , Yasuo Kawaguchi 3, 4
Affiliation
There exist two major types of striatum-targeting neocortical neurons, specifically, intratelencephalic (IT) neurons and pyramidal-tract (PT) neurons. Regarding their striatal projections, it was once suggested that IT axons are extended whereas PT axons are primarily focal. However, subsequent study with an increased number of well-stained extended axons concluded that such an apparent distinction was spurious due to limited sample size. Recent work using genetically labeled neurons reintroduced the differential spatial extent of the striatal projections of IT and PT neurons through population-level analyses, complemented by observations of single axons. However, quantitative IT vs. PT comparison of a large number of axons remained to be conducted. We analyzed the data of axonal end-points of 161 IT neurons and 33 PT neurons in the MouseLight database (http://ml-neuronbrowser.janelia.org/). The number of axonal end-points in the ipsilateral striatum exhibits roughly monotonically decreasing distributions in both neuron types. Excluding neurons with no ipsilateral end-point, the distributions of the logarithm of the number of ipsilateral end-points are considerably overlapped between IT and PT neurons, although the proportion of neurons having more than 50 ipsilateral end-points is somewhat larger in IT neurons than in PT neurons. Looking at more details, among IT subpopulations in the secondary motor area (MOs), layer 5 neurons and bilateral striatum-targeting layer 2/3 neurons, but not contralateral striatum-non-targeting layer 2/3 neurons, have a larger number of ipsilateral end-points than MOs PT neurons. We also found that IT ipsilateral striatal axonal end-points are on average more widely distributed than PT end-points, especially in the medial-lateral direction. These results indicate that IT and PT striatal axons differ in the frequencies and spatial extent of end-points while there are wide varieties within each neuron type.
中文翻译:
颅内和锥体束神经元的纹状体轴索差异化:MouseLight数据库中数据的分析。
存在两种主要的纹状体靶向新皮层神经元,特别是脑内(IT)神经元和锥体束(PT)神经元。关于其纹状体投射,曾经有人建议将IT轴突扩展,而PT轴突主要聚焦。然而,随后的研究中,增加数量的染色良好的延伸轴突得出结论,由于样本量有限,这种明显的区别是虚假的。通过基因组标记的神经元的最新工作通过人群水平分析,再加上对单个轴突的观察,重新引入了IT和PT神经元的纹状体投射的差异性空间范围。但是,仍需要进行大量轴突的定量IT与PT比较。我们在MouseLight数据库(http://ml-neuronbrowser.janelia.org/)中分析了161个IT神经元和33个PT神经元的轴突端点数据。同侧纹状体中轴突端点的数目在两种神经元类型中均表现出大致单调减少的分布。除没有同侧端点的神经元外,IT和PT神经元之间同侧端点数量的对数分布相当重叠,尽管IT神经元中具有50个以上同侧端点的神经元的比例稍大比PT神经元 查看更多详细信息,在次要运动区域(MOs)的IT子群体中,第5层神经元和双侧纹状体靶向层2/3神经元,但对侧纹状体非靶向层2/3神经元没有,具有比MOs PT神经元更大的同侧端点。我们还发现,IT同侧纹状体轴突端点平均比PT端点分布更广泛,尤其是在内侧-外侧方向上。这些结果表明,IT和PT纹状体轴突在端点的频率和空间范围上有所不同,而每种神经元类型内的变异却很大。
更新日期:2019-11-15
中文翻译:
颅内和锥体束神经元的纹状体轴索差异化:MouseLight数据库中数据的分析。
存在两种主要的纹状体靶向新皮层神经元,特别是脑内(IT)神经元和锥体束(PT)神经元。关于其纹状体投射,曾经有人建议将IT轴突扩展,而PT轴突主要聚焦。然而,随后的研究中,增加数量的染色良好的延伸轴突得出结论,由于样本量有限,这种明显的区别是虚假的。通过基因组标记的神经元的最新工作通过人群水平分析,再加上对单个轴突的观察,重新引入了IT和PT神经元的纹状体投射的差异性空间范围。但是,仍需要进行大量轴突的定量IT与PT比较。我们在MouseLight数据库(http://ml-neuronbrowser.janelia.org/)中分析了161个IT神经元和33个PT神经元的轴突端点数据。同侧纹状体中轴突端点的数目在两种神经元类型中均表现出大致单调减少的分布。除没有同侧端点的神经元外,IT和PT神经元之间同侧端点数量的对数分布相当重叠,尽管IT神经元中具有50个以上同侧端点的神经元的比例稍大比PT神经元 查看更多详细信息,在次要运动区域(MOs)的IT子群体中,第5层神经元和双侧纹状体靶向层2/3神经元,但对侧纹状体非靶向层2/3神经元没有,具有比MOs PT神经元更大的同侧端点。我们还发现,IT同侧纹状体轴突端点平均比PT端点分布更广泛,尤其是在内侧-外侧方向上。这些结果表明,IT和PT纹状体轴突在端点的频率和空间范围上有所不同,而每种神经元类型内的变异却很大。