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K31 as a novel marker for clear secretory cells in human eccrine sweat glands.
Journal of Molecular Histology ( IF 3.2 ) Pub Date : 2020-01-23 , DOI: 10.1007/s10735-020-09855-y
Lijie Du 1 , Xiang Zhang 1 , Liyun Chen 2 , Lei Zhang 3 , Haihong Li 1
Affiliation  

K31 was previously considered as one of the hair keratins. During a study on differential markers between hair follicles and eccrine sweat glands, we observed that K31 was expressed in eccrine sweat gland cells in a scattered pattern, similar to the distribution of dark or clear secretory cells. To investigate the precise cell localization of K31 in human eccrine sweat glands and find new marker for eccrine sweat gland cells, human skin samples were fixed, paraffined and sectioned. The serial sections were stained for K31, dark secretory cell marker gross cystic disease fluid protein 15 (GCDFP15) and clear secretory cell marker carbonic anhydrase II (CAII). The exact cell localization of K31 was detected by double immunofluorescence staining of K31 and a serial of cell-specific markers, and further by dual stain using a combination of periodic acid-Schiff (PAS) and immunofluorescence for K31 and GCDFP15. The expression pattern of K31-positive cells was similar to that of CAII-positive cells but was different from that of GCDFP15-positive staining in serial sections. Double immunofluorescent staining showed that K31-positive cells co-expressed K7 and CAII, but not S100P, α-SMA or GCDFP15. Dual stain by combined PAS and immunofluorescence showed that K31-positive cells are negative for PAS staining. We conclude that K31 is a previously unreported eccrine clear cell marker that allows for distinction between clear and dark secretory cells, as well as between secretory coils and ducts of eccrine sweat glands in human eccrine sweat glands.

中文翻译:

K31是人类内分泌汗腺中透明分泌细胞的新型标记物。

K31以前被认为是头发角蛋白之一。在研究毛囊和内分泌汗腺之间的差异标记时,我们观察到K31在内分泌汗腺细胞中以分散模式表达,类似于深色或透明分泌细胞的分布。为了研究K31在人内分泌汗腺细胞中的精确细胞定位并寻找内分泌汗腺细胞的新标志物,将人体皮肤样品固定,石蜡和切片。对连续切片进行K31染色,深色分泌细胞标记总囊性疾病液蛋白15(GCDFP15)和透明分泌细胞标记碳酸酐酶II(CAII)。通过K31的双重免疫荧光染色和一系列细胞特异性标记物检测到K31的确切细胞定位,然后通过高碘酸席夫(PAS)和免疫荧光的组合对K31和GCDFP15进行双重染色。在连续切片中,K31阳性细胞的表达模式与CAII阳性细胞相似,但与GCDFP15阳性染色不同。双重免疫荧光染色显示K31阳性细胞共表达K7和CAII,但不表达S100P,α-SMA或GCDFP15。结合PAS和免疫荧光法进行的双重染色显示,K31阳性细胞对PAS染色呈阴性。我们得出的结论是,K31是以前未报告的内分泌透明细胞标记物,可区分人类分泌性汗腺中的透明分泌细胞和深色分泌细胞,以及分泌线圈和内分泌汗腺导管之间的区别。K31阳性细胞的表达模式与CAII阳性细胞相似,但在连续切片中不同于GCDFP15阳性染色。双重免疫荧光染色显示K31阳性细胞共表达K7和CAII,但不表达S100P,α-SMA或GCDFP15。结合PAS和免疫荧光法进行的双重染色显示,K31阳性细胞对PAS染色呈阴性。我们得出的结论是,K31是以前未报告的内分泌透明细胞标记物,可区分人类分泌性汗腺中的透明分泌细胞和深色分泌细胞,以及分泌线圈和内分泌汗腺导管之间的区别。在连续切片中,K31阳性细胞的表达模式与CAII阳性细胞相似,但与GCDFP15阳性染色不同。双重免疫荧光染色显示K31阳性细胞共表达K7和CAII,但不表达S100P,α-SMA或GCDFP15。结合PAS和免疫荧光法进行的双重染色显示,K31阳性细胞对PAS染色呈阴性。我们得出的结论是,K31是以前未报告的内分泌透明细胞标记物,可区分人类分泌性汗腺中的透明分泌细胞和深色分泌细胞,以及分泌线圈和内分泌汗腺导管之间的区别。但不是S100P,α-SMA或GCDFP15。结合PAS和免疫荧光法进行的双重染色显示,K31阳性细胞对PAS染色呈阴性。我们得出的结论是,K31是以前未报告的内分泌透明细胞标记物,可区分人类分泌性汗腺中的透明分泌细胞和深色分泌细胞,以及分泌线圈和内分泌汗腺导管之间的区别。但不是S100P,α-SMA或GCDFP15。结合PAS和免疫荧光法进行的双重染色显示,K31阳性细胞对PAS染色呈阴性。我们得出的结论是,K31是以前未报告的内分泌透明细胞标记物,可区分人类分泌性汗腺中的透明分泌细胞和深色分泌细胞,以及分泌线圈和内分泌汗腺导管之间的区别。
更新日期:2020-01-23
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