There is a diverse population of stem cells in human breast milk that can be employed for therapeutic purposes as a reservoir of cells. The current study mainly aimed to determine the nature markers expressing on stem cells. For this aim, the expression of embryonic stem cell markers, as well as the expression of endothelial, mesenchymal, neural, and hematopoietic markers were evaluated by the flow cytometry analysis in fresh colostrum, breast milk, and cultured colostrum samples. The results showed that the embryonic (OCT4, SOX2, HLA-DR), hematopoietic (CD33, CD45, CD117), neural (CD133, Nestin), and mesenchymal (CD44, SCA1) stem cell markers present in colostrum had higher expression in comparison with their counterpart markers in fresh breast milk. The expression markers of stem cells in colostrum following a 2-week culture period were significantly increased compared with their counterpart markers in colostrum before the culture process. In the culture of breastmilk, cells were not observed adherent cells and colonies. Our findings form flow cytometry and cell culture suggest that the lactation stage could be one of the factors influencing the stem cell population and, consequently, the cultivation of breastmilk cells. The present study indicates that colostrum is a tremendous source of stem cells that could be applied in cell-based research.

中文翻译：

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人初乳和母乳干细胞的比较表型特征。

人类母乳中有各种各样的干细胞群，可作为细胞库用于治疗目的。目前的研究主要旨在确定在干细胞上表达的自然标志物。为此，通过流式细胞术分析在新鲜初乳、母乳和培养的初乳样品中评估胚胎干细胞标志物的表达，以及内皮、间充质、神经和造血标志物的表达。结果表明，初乳中存在的胚胎干细胞（OCT4、SOX2、HLA-DR）、造血干细胞（CD33、CD45、CD117）、神经干细胞（CD133、Nestin）和间充质干细胞（CD44、SCA1）标志物的表达量比较高。与新鲜母乳中的对应标记。与培养前初乳中的对应标志物相比，培养2周后初乳中干细胞的表达标志物显着增加。在母乳培养中，未观察到细胞贴壁细胞和集落。我们的流式细胞术和细胞培养结果表明，泌乳期可能是影响干细胞群的因素之一，从而影响母乳细胞的培养。本研究表明，初乳是干细胞的重要来源，可用于基于细胞的研究。我们的流式细胞术和细胞培养结果表明，泌乳期可能是影响干细胞群的因素之一，从而影响母乳细胞的培养。本研究表明，初乳是干细胞的重要来源，可用于基于细胞的研究。我们的流式细胞术和细胞培养结果表明，泌乳期可能是影响干细胞群的因素之一，从而影响母乳细胞的培养。本研究表明，初乳是干细胞的重要来源，可用于基于细胞的研究。