Figure 1: RNA ® Chromogenic (DAB) in situ hybridization assay (ISH) for Dickkopf-1 (DKK1) in prostate cancer. The sample is positive for peptidylprolyl cis-trans isomerase B (PPIB) and negative for bacterial dihydrodipicolinate reductase (dapB) RNA expression. Chose the letter that corresponds to the chromogenic RNA signal in Figure 1. Figure 2: DAPI-labeled, merged epi-fluorescence image of the same sample shown in Figure 1. No immunological or in situ hybridization assay was performed on this section. Blue, green and red wavelength channels are captured with matching camera settings. Identify the pigment producing the merged autofluorescence in Figure 2 and brightfield signal in Figure 1.

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图 1：前列腺癌中 Dickkopf-1 (DKK1) 的 RNA ® 显色 (DAB) 原位杂交测定 (ISH)。样品对肽基脯氨酰顺反异构酶 B (PPIB) 呈阳性，对细菌二氢吡啶二羧酸还原酶 (dapB) RNA 表达呈阴性。选择与图 1 中的显色 RNA 信号相对应的字母。 图 2：图 1 中显示的同一样品的 DAPI 标记的合并落射荧光图像。本节未进行免疫学或原位杂交检测。使用匹配的相机设置捕获蓝色、绿色和红色波长通道。确定产生图 2 中合并自发荧光的颜料和图 1 中的明场信号。