The purpose was to investigate a simultaneous biodegradation of the recalcitrant monoazo dye Reactive Orange 16 (RO16) in a mixed culture consisting of a biofilm of Pleurotus ostreatus–colonizing polyamide carrier and a suspension of the yeast Candida zeylanoides to see their biological interactions and possible synergistic action during degradation. Decolorization in the mixed culture was more effective than in the fungal monoculture, the respective decolorizations reaching 87.5% and 70% on day 11. The proliferation of yeast was reduced compared with the C. zeylanoides monoculture but enabled the yeast to participate in decolorization. The interaction of P. ostreatus with the yeast resulted in a gradual decrease of fungal manganese-dependent peroxidase (MnP) and laccase activities. Gas chromatography-mass spectrometry (GC-MS) analysis of the degradation products brought evidence that P. ostreatus split the dye molecule asymmetrically to provide 4-(ethenylsulfonyl) benzene whose concentration was much decreased in the mixed culture suggesting its increased metabolization in the presence of the yeast. In contrast, C. zeylanoides split the azo bond symmetrically producing the metabolites 4-(ethenylsulfonyl) aniline and α-hydroxybenzenepropanoic acid. Those metabolites were rapidly degraded in the mixed culture. A novel aspect is represented by the evidence of a mutual cooperative action of the fungal and yeast microorganisms in the mixed culture resulting in rapid decolorization and degradation of the dye.

目的是在混合培养物中研究顽固性单偶氮染料活性橙16（RO16）的同时生物降解，该培养物由平菇属生物膜–殖民化聚酰胺载体和酵母假单胞菌Zeylanoides悬浮液组成，以观察它们的生物学相互作用和可能的协同作用降解过程中的作用。混合培养物中的脱色比真菌单一培养物中的脱色更有效，在第11天各自的脱色分别达到87.5％和70％。与玉米淀粉鞘脂单培养相比，酵母的增殖有所降低，但能够使酵母参与脱色。平菇的相互作用酵母导致真菌锰依赖性过氧化物酶（MnP）和漆酶活性逐渐降低。降解产物的气相色谱-质谱（GC-MS）分析所带来的证据表明，平菇不对称分裂染料分子，以提供4-（乙烯基磺酰基）苯，其浓度在混合培养多下降表明其增加的代谢在存在酵母。相反，玉米C对称分解偶氮键，生成代谢物4-（乙烯基磺酰基）苯胺和α-羟基苯丙酸。这些代谢物在混合培养中迅速降解。一个新颖的方面是真菌和酵母微生物在混合培养物中相互协同作用的证据所代表，该相互作用导致染料快速脱色和降解。