Cyclophyllidean tapeworms obligatorily parasitize numerous mammalian species, including herbivores, domestic animals and humans, of which, the genera Taenia and Mesocestoides are well characterized. However, little is known about these parasitic infections in wild animals. This study aims to investigate the prevalence and distribution of Taenia sp. and Mesocestoides sp. in wild carnivores in Mongolia by identifying tapeworm species based on mtDNA analysis. The field survey was carried out in 2012-2013 in 19 provinces located in different ecological regions. A total of 405 fecal samples from wild carnivores were collected. Specific DNA markers in fecal samples was detected via copro-DNA analysis and tapeworm species were identified by DNA sequencing. From 27.7% (112/405) of samples, cox1 and 12S rRNA genes of tapeworms were amplified. Further, Taenia hydatigena (50.0%, 56/112) and two Mesocestoides species, including Mesocestoides sp.-1 (36.6%, 41/112) and Mesocestoides sp.-2 (13.4%, 15/112) were identified by DNA sequencing. The prevalence of T. hydatigena was 19.9% (27/136), 13.8% (23/167), 4.8% (3/62), and 7.5% (3/40) in wolves, red foxes, corsac foxes, and snow leopards, respectively. The prevalence of Mesocestoides sp.-1 was 14.7% (20/136), 9% (15/167), 9.7% (6/62) in wolves, red foxes, and corsac foxes, while the prevalence of Mesocestoides sp.-2 was 4.4% (6/136), 1.8% (3/167), 3.2% (2/62), and 10.0% (4/40) in wolves, red foxes, corsac foxes, and snow leopards, respectively. T. hydatigena was found throughout all ecological regions, while Mesocestoides sp.-1 was in the mountain taiga, forest-steppe, steppe, desert-steppe, and desert, and Mesocestoides sp.-2 in the alpine, forest-steppe, steppe, and desert-steppe ecoregions. This study revealed the prevalence and distribution of cyclophyllidean tapeworms in wild carnivores in Mongolia; while also confirming that wolves, red foxes, corsac foxes, and snow leopards serve as definitive hosts for unidentified Mesocestoides species.

中文翻译：

---

基于蒙古野生食肉动物的propro-DNA分析，对hy虫和Me虫的分子鉴定。

环叶螺tape虫会寄生许多哺乳动物，包括草食动物，家畜和人类，其中Ta虫属和Me虫属具有很好的特征。但是，对于野生动物中的这些寄生虫感染知之甚少。这项研究旨在调查investigate虫的流行和分布。和Mesocestoides sp。mtDNA分析通过鉴定tape虫物种，在蒙古的野生食肉动物中发现它们。实地调查于2012-2013年在不同生态区域的19个省进行。总共从野生食肉动物中收集了405份粪便样品。通过copro-DNA分析检测粪便样品中的特定DNA标记，并通过DNA测序鉴定tape虫种类。从27.7％（112/405）的样本中，扩增了tape虫的cox1和12S rRNA基因。进一步，通过DNA测序鉴定出牛带状en虫（Taenia hydatigena）（50.0％，56/112）和两个中so类，包括中so类sp.-1（36.6％，41/112）和中so类sp.-2（13.4％，15/112）。在狼，赤狐，科萨克斯狐狸和雪中，T。hydatigena的患病率分别为19.9％（27/136），13.8％（23/167），4.8％（3/62）和7.5％（3/40）豹，分别。Mesocestoides sp.-1的患病率在狼，赤狐和corsac狐狸中分别为14.7％（20/136），9％（15/167），9.7％（6/62），而Mesocestoides sp.-在狼，赤狐，科萨克斯狐狸和雪豹中，2分别是4.4％（6/136），1.8％（3/167），3.2％（2/62）和10.0％（4/40）。在所有生态区中都发现了T. hydatigena，而Mesocestoides sp.-1分布在针叶林，针叶林草原，草原，沙漠草原和沙漠，而Mesocestoides sp.-2分布于高山，森林草原，草原和沙漠草原生态区。这项研究揭示了蒙古野生食肉动物中环叶利德tape虫的流行和分布。同时还确认了狼，赤狐，科萨克斯狐狸和雪豹是身份不明的Mesocestoides物种的权威宿主。