Objective. BioCartilage is a desiccated, particulated cartilage allograft used for repair of focal cartilage defects. It is mixed with a biologic such as bone marrow concentrate (BMC), pressed into a contained defect, and sealed with fibrin glue. The objective of this study was to assess if BioCartilage could serve as a bioactive scaffold by affecting cellular adhesion, cellular migration, or the release interleukin-1 receptor antagonist protein (IL-1RA), and to identify its full proteomic makeup. Design. Cartilage explants were used to model confined defects. BioCartilage was mixed with BMC, grafted into defects, and sealed with 1 of 5 fibrin glues. Constructs were cultured for 24 or 48 hours and then processed for live/dead microscopy. Chondrocyte and mesenchymal stem cell (MSC) adhesion on BioCartilage was assessed using scanning electron microscopy. Conditioned medium from cultures and the biologics used in the study were assayed for IL-1RA. The protein footprint of BioCartilage was determined using bottom-up proteomics. Results. BioCartilage supported chondrocyte and MSC attachment within 24 hours, and cell viability was retained in all constructs at 24 and 48 hours. Fibrin glue did not inhibit cell attachment. BMC had the highest concentration of IL-1RA. Proteomics yielded 254 proteins, including collagens, proteoglycans, and several bioactive proteins with known anabolic roles including cartilage oligomeric matrix protein. Conclusions. This study suggests that BioCartilage has the chemical composition and architecture to support cell adherence and migration and to provide bioactive proteins, which together should have biologics advantages in cartilage repair beyond its role as a scaffold.

中文翻译：

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使用生物软骨支架修复软骨的生物学机制：细胞粘附/迁移和生物活性蛋白。

客观的。BioCartilage 是一种干燥的微粒软骨同种异体移植物，用于修复局部软骨缺损。它与诸如骨髓浓缩物 (BMC) 之类的生物制剂混合，压入包含的缺陷中，并用纤维蛋白胶密封。本研究的目的是评估 BioCartilage 是否可以通过影响细胞粘附、细胞迁移或释放白介素-1 受体拮抗蛋白 (IL-1RA) 来充当生物活性支架，并确定其完整的蛋白质组构成。设计。软骨外植体用于模拟受限缺陷。BioCartilage 与 BMC 混合，移植到缺损处，并用 5 种纤维蛋白胶中的 1 种进行密封。将构建体培养 24 或 48 小时，然后进行活/死显微镜检查。使用扫描电子显微镜评估生物软骨上的软骨细胞和间充质干细胞 (MSC) 粘附。测定来自培养物的条件培养基和研究中使用的生物制剂的 IL-1RA。BioCartilage 的蛋白质足迹是使用自下而上的蛋白质组学确定的。结果。BioCartilage 在 24 小时内支持软骨细胞和 MSC 附着，并且在 24 和 48 小时内所有构建体中都保留了细胞活力。纤维蛋白胶不抑制细胞附着。BMC具有最高浓度的IL-1RA。蛋白质组学产生了 254 种蛋白质，包括胶原蛋白、蛋白聚糖和几种具有已知合成代谢作用的生物活性蛋白质，包括软骨寡聚基质蛋白。结论。