Abstract

The use of inorganic copper in feed is hazardous. As probiotics of animals, Candida utilis can absorb copper ions and transform them to organic copper. This study aimed to domesticate the ability of C. utilis (CICC 32211) to absorb and transform copper ions. So, C. utilis (CICC 32211) was cultured in media with different concentrations of copper ions for 24, 48 and 72 h to identify the optimum copper ion concentration. C. utilis (CICC 32211) strains were domesticated for three generations, then the absorption and distribution of copper ions in the yeast cells were studied. We found that the optimum concentration of copper ions was 110 µg/mL. After 48 h, the number of yeast cells was low, but copper ion absorption efficiency was maximized (43.83%). Most of the enriched copper ions were distributed in the yeast cell wall (up to 30.58% when grown in the medium with 110 µg/mL copper ions), while the intracellular copper ion content was low (2.095%). High concentrations of copper ions affected the morphological structure, element content and distribution of yeast cells.

摘要

在饲料中使用无机铜是危险的。作为动物的益生菌，念珠菌可以吸收铜离子并将其转化为有机铜。本研究旨在驯化C. utilis (CICC 32211) 吸收和转化铜离子的能力。因此，将C. utilis (CICC 32211) 在具有不同铜离子浓度的培养基中培养 24、48 和 72 小时，以确定最佳铜离子浓度。C. utilis(CICC 32211)菌株被驯化三代，然后研究铜离子在酵母细胞中的吸收和分布。我们发现铜离子的最佳浓度为 110 µg/mL。48 小时后，酵母细胞数量减少，但铜离子吸收效率最大化（43.83%）。大多数富集的铜离子分布在酵母细胞壁中（在含有 110 µg/mL 铜离子的培养基中生长时高达 30.58%），而细胞内铜离子含量较低（2.095%）。高浓度的铜离子影响酵母细胞的形态结构、元素含量和分布。