Cisplatin (CP) is an antineoplastic drug; however, owing to its nephrotoxicity, its clinical use is limited. We investigated whether vitexilactone (vitex) is a safe and effective treatment for CP induced kidney injury. We allocated Sprague-Dawley rats into six groups: control group, low dose-high dose vitex groups (40 and 80 mg/kg vitex for 6 days before administration of CP), CP group (single 6 mg/kg dose on day 6) and CP + low dose vitex-CP + high dose vitex group (40 and 80 mg/kg vitex for 6 days, and a single 6 mg/kg dose of CP on day 6. Rats were euthanized 5 days after CP treatment. After exposure to CP and/or vitex, total oxidative stress and total antioxidant status were assessed. The histology of the kidney was examined using hematoxylin and eosin, and periodic acid-Schiff. We used immunohistochemical and fluorescence staining to detect expression of caspase-3. We also measured blood urea nitrogen, uric acid and creatinine levels. Nephroprotective effects of vitex were associated with decreased serum toxicity markers and increased antioxidant activity. Vitex also reduced the expression of the apoptosis marker, caspase-3. Treatment with CP increased blood urea nitrogen, uric acid, creatinine levels and total antioxidant status, and decreased total antioxidant status compared to the control group. Use of vitex for protection from CP induced nephrotoxicity appears to be a safe and efficacious alternative for treatment of kidney injury.

顺铂（CP）是抗肿瘤药；然而，由于其肾毒性，其临床用途受到限制。我们调查了维特西内酯（vitex）是否是治疗CP致肾损伤的安全有效方法。我们将Sprague-Dawley大鼠分为6组：对照组，低剂量-高剂量胎膜早破组（CP给药前6天分别为40和80 mg / kg胎膜早孕），CP组（第6天单次6 mg / kg剂量）以及CP +低剂量vitex-CP +高剂量vitex组（分别以40和80 mg / kg的剂量连续6天，并在第6天注入6 mg / kg的单剂量CP）。CP处理后5天对大鼠实施安乐死。对CP和/或胎膜，评估总氧化应激和总抗氧化状态，使用苏木精和曙红，高碘酸-席夫（Schiff）检查肾脏的组织学。我们使用免疫组化和荧光染色来检测caspase-3的表达。我们还测量了血液中的尿素氮，尿酸和肌酐水平。胎膜的肾保护作用与降低血清毒性标志物和增加抗氧化活性有关。Vitex还降低了凋亡标记caspase-3的表达。与对照组相比，CP治疗可增加血液尿素氮，尿酸，肌酐水平和总抗氧化剂状态，并降低总抗氧化剂状态。使用保护膜免受CP诱导的肾毒性似乎是治疗肾损伤的一种安全有效的替代方法。胎膜的肾保护作用与降低血清毒性标志物和增加抗氧化活性有关。Vitex还降低了凋亡标记caspase-3的表达。与对照组相比，CP治疗可增加血液尿素氮，尿酸，肌酐水平和总抗氧化剂状态，并降低总抗氧化剂状态。使用保护膜免受CP诱导的肾毒性似乎是治疗肾损伤的一种安全有效的替代方法。胎膜的肾保护作用与降低血清毒性标志物和增加抗氧化活性有关。Vitex还降低了凋亡标记caspase-3的表达。与对照组相比，CP治疗可增加血液尿素氮，尿酸，肌酐水平和总抗氧化剂状态，并降低总抗氧化剂状态。使用保护膜免受CP诱导的肾毒性似乎是治疗肾损伤的一种安全有效的替代方法。