Mechanisms of immune activation in effector cells during Haemonchus contortus infection in sheep are currently unknown. Microarray experiments have been performed on tissues of H. contortus infected sheep of varying parasite resistance during early and late points of infection, but not in immune effector cells. The purpose of this study was to compare early gene activation in peripheral blood mononuclear cells (PBMC) from primed parasite susceptible (Suffolk) and resistant (St. Croix) sheep in response to H. contortus larval antigen (HcLA). Peripheral Blood Mononuclear Cells were cultured for 6 hours with HcLA and RNA-Sequencing was performed. St. Croix PBMC upregulated 499 unique genes in response to HcLA while Suffolk PBMC upregulated 130 unique genes and 25 genes were shared between the two breeds. St. Croix PBMC had increased expression of genes associated with immune function, signal transduction, response to stress and others. In addition, while mechanisms of innate recognition of H. contortus are unknown, multiple pattern recognition receptors were found to be upregulated in St. Croix PBMC cultured with HcLA and none were found to be upregulated in Suffolk PBMC. These patterns of immune gene activation may contribute to St. Croix's rapid response and ability to resist H. contortus infection.

中文翻译：

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暴露于捻转血矛线虫幼虫抗原后绵羊PBMC的RNA测序。

目前尚不清楚绵羊羊膜弯曲杆菌感染期间效应细胞中免疫激活的机制。已经在感染的早期和晚期对寄生虫抗药性不同的经Contortus感染的绵羊的组织进行了微阵列实验，但未在免疫效应细胞中进行。这项研究的目的是比较来自对初生寄生虫敏感（萨福克）和抗性（圣克鲁瓦）羊的对弓形虫幼虫抗原（HcLA）反应的外周血单核细胞（PBMC）的早期基因激活。将外周血单核细胞与HcLA培养6小时，然后进行RNA测序。St. Croix PBMC响应HcLA上调499个独特基因，而萨福克PBMC上调130个独特基因，而两个品种之间共有25个基因。圣 Croix PBMC具有与免疫功能，信号转导，对压力反应等相关的基因表达增加。此外，虽然尚不了解先天认认捻转嗜血杆菌的机制，但在用HcLA培养的St. Croix PBMC中发现了多种模式识别受体上调，而在Suffolk PBMC中未发现上调模式识别受体。免疫基因激活的这些模式可能有助于圣克鲁瓦的快速反应和抵抗弯曲杆菌感染的能力。