Primary Ovarian Insufficiency (POI) is one of the main diseases causing female infertility that occurs in about 1% of women between 30-40 years of age. There are few effective methods for the treatment of women with POI. In the past few years, stem cell-based therapy as one of the most highly investigated new therapies has emerged as a promising strategy for the treatment of POI. Human pluripotent stem cells (hPSCs) can self-renew indefinitely and differentiate into any type of cell. Human Embryonic Stem Cells (hESCs) as a type of pluripotent stem cells are the most powerful candidate for the treatment of POI. Human-induced Pluripotent Stem Cells (hiPSCs) are derived from adult somatic cells by the treatment with exogenous defined factors to create an embryonic-like pluripotent state. Both hiPSCs and hESCs can proliferate and give rise to ectodermal, mesodermal, endodermal, and germ cell lineages. After ovarian stimulation, the number of available oocytes is limited and the yield of total oocytes with high quality is low. Therefore, a robust and reproducible in-vitro culture system that supports the differentiation of human oocytes from PSCs is necessary. Very few studies have focused on the derivation of oocyte-like cells from hiPSCs and the details of hPSCs differentiation into oocytes have not been fully investigated. Therefore, in this review, we focus on the differentiation potential of hPSCs into human oocyte-like cells.

原发性卵巢功能不全（POI）是引起女性不育的主要疾病之一，大约30％至40岁之间的女性中约有1％会发生这种疾病。治疗POI女性的有效方法很少。在过去的几年中，基于干细胞的疗法已成为研究最广的新疗法之一，已成为治疗POI的一种有前途的策略。人多能干细胞（hPSC）可以无限期自我更新并分化为任何类型的细胞。人胚胎干细胞（hESCs）是一种多能干细胞，是治疗POI的最有力候选者。人类诱导的多能干细胞（hiPSC）来源于成人体细胞，通过外源定义因子进行处理，从而形成胚胎样多能状态。hiPSC和hESC均可增殖并产生外胚层，中胚层，内胚层和生殖细胞谱系。卵巢刺激后，可用卵母细胞的数量受到限制，高质量卵母细胞的总产量较低。因此，有必要提供一种强大且可重现的体外培养系统，以支持人卵母细胞从PSC分化。很少有研究集中于从hiPSCs衍生卵母细胞样细胞，而hPSCs分化为卵母细胞的细节尚未得到充分研究。因此，在这篇综述中，我们集中于hPSCs分化为人卵母细胞样细胞的潜力。必须有一个强大且可重现的体外培养系统来支持人类卵母细胞从PSC分化。很少有研究集中于从hiPSCs衍生卵母细胞样细胞，而hPSCs分化为卵母细胞的细节尚未得到充分研究。因此，在这篇综述中，我们集中于hPSCs分化为人卵母细胞样细胞的潜力。必须有一个强大且可重现的体外培养系统来支持人类卵母细胞从PSC分化。很少有研究集中于从hiPSCs衍生卵母细胞样细胞，而hPSCs分化为卵母细胞的细节尚未得到充分研究。因此，在这篇综述中，我们集中于hPSCs向人卵母细胞样细胞的分化潜能。