Acinetobacter baumannii is an important opportunistic pathogen that shows resistance to cephalosporins, penicillins, carbapenems, fluoroquinolones, and aminoglycosides, the multiresistance being associated with its ability to form biofilms in clinical environments. The aim of this study was to determine biofilm formation and its potential association with genes involved in antibiotic resistance mechanisms of A. baumannii isolates of different clinical specimens. We demonstrated 100% of the A. baumannii isolates examined to be multidrug resistant (MDR), presenting a 73.3% susceptibility to cefepime and a 53.3% susceptibility to ciprofloxacin. All A. baumannii isolates were positive for bla_OXA-51, 33.3% being positive for bla_OXA-23 and ISAba1, and 73.3% being positive for gyrA. We found 86.6% of A. baumannii strains to be low-grade biofilm formers and 13.3% to be biofilm negative; culturing on Congo red agar (CRA) plates revealed that 73.3% of the A. baumannii isolates to be biofilm producers, while 26.6% were not. These properties, combined with the role of A. baumannii as a nosocomial pathogen, increase the probability of A. baumannii causing nosocomial infections and outbreaks as a complication during therapeutic treatments and emphasize the need to control A. baumannii biofilms in hospital environments.

中文翻译：

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耐多药鲍曼不动杆菌中氟喹诺酮和碳青霉烯抗性基因的生物膜形成和检测。

鲍曼不动杆菌是一种重要的机会病原体，对头孢菌素，青霉素，碳青霉烯，氟喹诺酮和氨基糖苷显示出耐药性，其多重耐药性与其在临床环境中形成生物膜的能力有关。这项研究的目的是确定生物膜的形成及其与不同临床标本中鲍曼不动杆菌分离物的抗生素抗性机制相关基因的潜在关联。我们证明100％的鲍曼不动杆菌分离株具有多重耐药性（MDR），对头孢吡肟的敏感性为73.3％，对环丙沙星的敏感性为53.3％。鲍曼不动杆菌的所有分离株均为bla _OXA-51阳性，其中bla _OXA-23和IS Aba1阳性的占33.3％，而gyrA阳性的占73.3％。我们发现86.6％的鲍曼不动杆菌菌株是低级生物膜形成者，而13.3％是生物膜阴性。在刚果红琼脂（CRA）平板上培养表明，鲍曼不动杆菌的73.3％分离物是生物膜生产者，而26.6％不是。这些特性与鲍曼不动杆菌作为医院病原体的作用相结合，增加了鲍曼不动杆菌在治疗过程中作为并发症引起医院内感染和暴发的可能性，并强调需要在医院环境中控制鲍曼不动杆菌生物膜。