Y-box binding proteins are DNA- and RNA-binding proteins with an evolutionarily ancient and conserved cold shock domain. The Y-box binding protein 1 (YB-1) is the most studied due to its abundance in somatic cells. YB-1 is involved in a variety of cellular processes, including proliferation, differentiation and stress response. Here, using Ribo-Seq and RIP-Seq we confirm that YB-1 binds a wide range of mRNAs and globally acts as a translation inhibitor. Surprisingly, YBX1 knockout results in only minor alterations in the expression of other genes, mostly caused by changes in RNA abundance. But YB-3 mRNA is an exception: it is better translated in the absence of YB-1, thereby producing an increased amount of YB-3 and thus suggesting that its synthesis is under YB-1 negative control. We have shown that the set of mRNAs bound to YB-3 is strikingly similar to that of YB-1, and that the mRNA-binding by YB-3 is enhanced in the absence of YB-1, resulting in a similar global reduction of translation of bound mRNAs in YB-1-null cells. Thus, YB-3 acts as a substitute for YB-1 in mRNA binding and, probably, in global translational control.

中文翻译：

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YB-3在整体mRNA结合中替代YB-1。

Y-box结合蛋白是具有进化上古老且保守的冷激域的DNA和RNA结合蛋白。由于Y盒结合蛋白1（YB-1）在体细胞中含量很高，因此研究最多。YB-1参与多种细胞过程，包括增殖，分化和应激反应。在这里，我们使用Ribo-Seq和RIP-Seq证实YB-1结合了广泛的mRNA，并且整体上充当翻译抑制剂。出人意料的是，YBX1基因敲除仅导致其他基因表达的微小变化，主要是由RNA丰度的变化引起的。但是YB-3 mRNA是一个例外：在没有YB-1的情况下翻译效果更好，因此产生的YB-3量增加，因此表明其合成处于YB-1阴性对照之下。我们已经表明，与YB-3结合的mRNA的集合与YB-1极为相似，并且在没有YB-1的情况下，由YB-3的mRNA结合得到增强，从而导致类似的整体减少。 YB-1- null细胞中结合的mRNA的翻译。因此，YB-3在mRNA结合中，可能在全局翻译控制中，可以替代YB-1。