We investigated the protective effect of iloprost against ischemia/reperfusion (I/R) injury in rat ovary. We used 32 female Sprague-Dawley rats randomly allocated to four experimental groups: sham, ischemia, I/R and I/R + iloprost. Ovarian torsion was established in all rats except the sham group. The torsion group was exposed to ischemia for 3 h. The detorsion group was exposed to 3 h ischemia applied + 3 h reperfusion. The detorsion + iloprost group was exposed to ischemia for 3 h + reperfusion for 3 h + intravenous (IV) iloprost infusion for 60 min starting at the beginning of reperfusion. Ovaries were removed and prepared for histopathological evaluation. Reduced glutathione (GSH) and malondialdehyde (MDA) were measured in the blood. The total histopathological injury score and MDA level of the ischemia group were significantly higher than for the sham group. Ovarian injury score and MDA level following I/R increased compared to the ischemia group. Iloprost administration reduced the total injury score and MDA level. The GSH level was higher in the I/R + iloprost group than in the I/R group. We concluded that IV iloprost administration reduces I/R injury in rat ovarian tissue.

我们调查了伊洛前列素对大鼠卵巢缺血/再灌注（I / R）损伤的保护作用。我们使用32只雌性Sprague-Dawley大鼠随机分配到四个实验组：假手术，局部缺血，I / R和I / R +伊洛前列素。除假手术组外，所有大鼠均产生卵巢扭转。扭转组暴露于缺血3小时。扭曲组暴露于3 h局部缺血+ 3 h再灌注。从开始再灌注开始，将扭曲+伊洛前列素组缺血3小时，再灌注3小时，再静脉注射伊洛前列素（IV）60分钟。摘除卵巢并准备进行组织病理学评估。在血液中检测到还原型谷胱甘肽（GSH）和丙二醛（MDA）。缺血组的总组织病理学损伤评分和MDA水平显着高于假手术组。与缺血组相比，I / R后的卵巢损伤评分和MDA水平升高。服用伊洛前列素可降低总损伤评分和MDA水平。I / R +伊洛前列素组的GSH水平高于I / R组。我们得出的结论是，静脉注射伊洛前列素可减少大鼠卵巢组织的I / R损伤。