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Isolation of culturable mycota from Argentinean soils exposed or not-exposed to pesticides and determination of glyphosate tolerance of fungal species in media supplied with the herbicide.
Revista Argentina de Microbiología ( IF 1.9 ) Pub Date : 2020-01-09 , DOI: 10.1016/j.ram.2019.11.003
Melisa Egle Aluffi 1 , Cecilia Soledad Carranza 1 , Nicolás Benito 1 , Karen Magnoli 1 , Carina Elizabeth Magnoli 1 , Carla Lorena Barberis 1
Affiliation  

The current agricultural system has led to the development of glyphosate (GP)-resistant weeds, causing an increase in GP doses and applications. Native mycota of pesticide-contaminated sites are the major source of pesticide-degrading microorganisms. The aims of the present study were to isolate the GP-tolerant culturable mycota in two soils with different pesticide exposure from Córdoba, Argentina, and to evaluate the growth parameters in native fungal isolates in the presence of GP and the effective dose that caused 50% growth reduction. The results showed that the genera Fusarium, Aspergillus, Mucor, Penicillium and Sterilia were the prevalent fungi isolated from soils both exposed and not-exposed to pesticides. The highest value (>100 mM) of effective concentration of herbicide that caused 50% growth inhibition (EC50), was found for Trichoderma isolates. Sterilia spp. had EC50 values of 100 mM, while Aspergillus spp. and Mucor had EC50 values between 50 and 100 mM. The growth rate evaluation varied according to the isolates and GP concentrations. The data showed that all Aspergillus spp., Trichoderma spp., Mucor and three Sterilia spp. had the best growth performance in media supplied with GP after a variable acclimation period. This study provides valuable data for further studies that would allow to know the metabolic capacity of these fungal species that can be potential candidates for GP removal from contaminated environments.



中文翻译:

从暴露于或未暴露于杀虫剂的阿根廷土壤中分离出可培养的真菌,并在提供除草剂的培养基中测定真菌物种的草甘膦耐受性。

当前的农业系统导致了抗草甘膦 (GP) 杂草的发展,导致 GP 剂量和应用的增加。农药污染场地的原生真菌是农药降解微生物的主要来源。本研究的目的是在阿根廷科尔多瓦不同农药暴露的两种土壤中分离耐 GP 的可培养真菌,并评估在 GP 存在下本地真菌分离株的生长参数和导致 50%增长减少。结果表明,镰刀菌属曲霉属、毛霉属青霉属和不菌属是从暴露于和未暴露于杀虫剂的土壤中分离出的流行真菌。对于木霉属分离株,发现导致 50% 生长抑制 (EC50) 的除草剂有效浓度的最高值 (>100 mM) 。不育菌属 具有 100 mM 的 EC50 值,而曲霉属。和毛霉有50和100mM之间的EC 50个值。生长速率评估根据分离株和 GP 浓度而变化。数据显示,所有曲霉属、木霉属、毛霉属和三种无菌属 在可变驯化期后,在提供 GP 的培养基中具有最佳的生长性能。这项研究为进一步研究提供了有价值的数据,可以了解这些真菌物种的代谢能力,这些真菌物种可能是从受污染环境中去除 GP 的潜在候选者。

更新日期:2020-01-09
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