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Uniform spheroid formation on a laboratory-made, low cell attachment surface consisting of a chitin sheet.
Bioscience, Biotechnology, and Biochemistry ( IF 1.6 ) Pub Date : 2020-01-11 , DOI: 10.1080/09168451.2020.1714423
Yusaku Inubushi 1 , Akira Tachibana 1
Affiliation  

We utilized the reaction of chitosan with acetic anhydride to form a chitin gel. This gel was then dried, which formed a chitin sheet. The procedure was extremely easy for a biologist unfamiliar with materials engineering. Spheroids derived from HEK293T cells were formed on the chitin sheet, because the spheroids slightly attached and slowly moved on the chitin sheet.

中文翻译:

在由几丁质片组成的实验室制造的低细胞附着表面上形成均匀的球体。

我们利用壳聚糖与乙酸酐的反应形成了几丁质凝胶。然后将该凝胶干燥,形成几丁质片。对于不熟悉材料工程的生物学家来说,该过程非常容易。在甲壳质片上形成了源自HEK293T细胞的球体,因为这些球体在甲壳质片上略微附着并缓慢移动。
更新日期:2020-04-20
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