Invasive Streptococcus pyogenes diseases represent the most severe form of infection produced by this microorganism. Early diagnosis and treatment are important, due to its potential severity. Etiological confirmation of invasive infection is performed by culture, which takes between 18 and 48 h. We tested a rapid immunochromatographic assay directly from clinical samples from normally sterile sites and positive blood culture bottles when positive cocci chains were observed by Gram staining. Eighty samples were analyzed. The rapid test was positive in 35 samples: in 34 of them S. pyogenes was confirmed by culture. The immunochromatographic method showed 97.1% sensitivity and 97.8% specificity. The strept A® immunochromatographic rapid test allows to obtain reliable results in less than 10 min and is accessible to any microbiology laboratory. This study demonstrates the potential use of a rapid immunochromatographic method directly from clinical samples and positive blood cultures.

侵袭性化脓性链球菌疾病是这种微生物产生的最严重的感染形式。由于其潜在的严重性，早期诊断和治疗很重要。侵入性感染的病因确认是通过培养进行的，需要 18 到 48 小时。当通过革兰氏染色观察到阳性球菌链时，我们直接从来自正常无菌部位和阳性血培养瓶的临床样本中测试了快速免疫色谱分析。分析了八十个样品。35 个样本的快速检测呈阳性：其中 34 个为化脓性链球菌经培养证实。免疫层析方法显示出 97.1% 的灵敏度和 97.8% 的特异性。链球菌 A® 免疫层析快速检测允许在不到 10 分钟的时间内获得可靠的结果，并且可供任何微生物实验室使用。这项研究证明了直接从临床样本和阳性血培养物中快速免疫层析方法的潜在用途。