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Cortisol urinary metabolites in dogs with hypercortisolism, congestive heart failure, and healthy dogs: pilot investigation.
The Journal of Veterinary Diagnostic Investigation ( IF 1.5 ) Pub Date : 2020-01-10 , DOI: 10.1177/1040638719899645
Ester Quilez 1 , Richard K Burchell 2 , Eric B Thorstensen 3 , Karin Weidgraaf 4 , Stacey E Parbhu 5 , Nicolas Lopez-Villalobos 4 , Arnon Gal 6
Affiliation  

Nonadrenal diseases (NAD), including congestive heart failure (CHF), can affect the conversion of cortisone to cortisol favoring the production of cortisol's urinary downstream metabolites 5α/5β-tetrahydrocortisol (THF) relative to tetrahydrocortisone (THE). We hypothesized that healthy dogs would have lower urinary levels of cortisol, cortisone, THF, and THE than dogs with hypercortisolism (HC) or CHF, and the latter would have higher urinary levels of THF and lower THE than dogs with HC. Four, 9, and 8 dogs with HC, CHF, and normal health, respectively, were included in a pilot prospective cross-sectional study. A single morning voided urine sample was analyzed for urinary cortisol metabolites by liquid chromatography-mass spectrometry. The percentages of conjugated urinary metabolites were significantly higher in dogs with CHF than in healthy dogs (p = 0.001), and not different in HC dogs (p = 0.07). Log-transformed urine cortisol metabolites-to-creatinine ratios in healthy dogs were significantly lower than the 2 other groups (p < 0.001). The urinary free THE:THF ratio was significantly higher (p < 0.001) than the urinary total and conjugated THE:THF ratios. Health status did not affect the total, conjugated, and free THE:THF ratios (p = 0.61). Additional studies are needed to investigate differences in cortisol metabolites between dogs with HC and NAD to accurately discriminate between the groups.

中文翻译:

患有皮质醇增多症、充血性心力衰竭和健康犬的犬的皮质醇尿代谢物:初步调查。

包括充血性心力衰竭 (CHF) 在内的非肾上腺疾病 (NAD) 会影响可的松向皮质醇的转化,相对于四氢可的松 (THE),有利于皮质醇的尿液下游代谢物 5α/5β-四氢皮质醇 (THF) 的产生。我们假设健康犬的尿皮质醇、可的松、THF 和 THE 水平低于患有高皮质醇症 (HC) 或 CHF 的犬,而后者的尿 THF 水平比患有 HC 的犬低。分别有 4 只、9 只和 8 只患有 HC、CHF 和正常健康的狗被纳入一项试点前瞻性横断面研究。通过液相色谱-质谱法分析了一个早晨排尿的尿样中的尿皮质醇代谢物。CHF 犬的结合尿代谢物百分比显着高于健康犬(p = 0.001),而 HC 犬无差异(p = 0.07)。健康犬的对数转换后的尿皮质醇代谢物与肌酐的比率显着低于其他 2 组 (p < 0.001)。无尿THF:THF比率显着高于(p < 0.001)尿总量和结合THF:THF比率。健康状况不影响总、共轭和游离 THE:THF 比率 (p = 0.61)。需要进一步的研究来调查患有 HC 和 NAD 的狗之间皮质醇代谢物的差异,以准确区分两组。健康犬的对数转换后的尿皮质醇代谢物与肌酐的比率显着低于其他 2 组 (p < 0.001)。无尿THF:THF比率显着高于(p < 0.001)尿总量和结合THF:THF比率。健康状况不影响总、共轭和游离 THE:THF 比率 (p = 0.61)。需要进一步的研究来调查患有 HC 和 NAD 的狗之间皮质醇代谢物的差异,以准确区分两组。健康犬的对数转换后的尿皮质醇代谢物与肌酐的比率显着低于其他 2 组 (p < 0.001)。无尿THF:THF比率显着高于(p < 0.001)尿总量和结合THF:THF比率。健康状况不影响总、共轭和游离 THE:THF 比率 (p = 0.61)。需要进一步的研究来调查患有 HC 和 NAD 的狗之间皮质醇代谢物的差异,以准确区分两组。
更新日期:2020-01-10
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