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Dynamics of human monocytes and airway macrophages during healthy aging and after transplant
The Journal of Experimental Medicine Pub Date : 2020-01-09 , DOI: 10.1084/jem.20191236 Adam J Byrne 1, 2 , Joseph E Powell 3, 4 , Brendan J O'Sullivan 5 , Patricia P Ogger 1 , Ashley Hoffland 1, 2 , James Cook 6 , Katie L Bonner 1, 6 , Richard J Hewitt 6 , Simone Wolf , Poonam Ghai 1 , Simone A Walker 1 , Samuel W Lukowski , Philip L Molyneaux 6 , Sejal Saglani 2, 6 , Daniel C Chambers 5 , Toby M Maher 6 , Clare M Lloyd 2
The Journal of Experimental Medicine Pub Date : 2020-01-09 , DOI: 10.1084/jem.20191236 Adam J Byrne 1, 2 , Joseph E Powell 3, 4 , Brendan J O'Sullivan 5 , Patricia P Ogger 1 , Ashley Hoffland 1, 2 , James Cook 6 , Katie L Bonner 1, 6 , Richard J Hewitt 6 , Simone Wolf , Poonam Ghai 1 , Simone A Walker 1 , Samuel W Lukowski , Philip L Molyneaux 6 , Sejal Saglani 2, 6 , Daniel C Chambers 5 , Toby M Maher 6 , Clare M Lloyd 2
Affiliation
The ontogeny of airway macrophages (AMs) in human lung and their contribution to disease are poorly mapped out. In mice, aging is associated with an increasing proportion of peripherally, as opposed to perinatally derived AMs. We sought to understand AM ontogeny in human lung during healthy aging and after transplant. We characterized monocyte/macrophage populations from the peripheral blood and airways of healthy volunteers across infancy/childhood (2–12 yr), maturity (20–50 yr), and older adulthood (>50 yr). Single-cell RNA sequencing (scRNA-seq) was performed on airway inflammatory cells isolated from sex-mismatched lung transplant recipients. During healthy aging, the proportions of blood and bronchoalveolar lavage (BAL) classical monocytes peak in adulthood and decline in older adults. scRNA-seq of BAL cells from lung transplant recipients indicates that after transplant, the majority of AMs are recipient derived. These data show that during aging, the peripheral monocyte phenotype is consistent with that found in the airways and, furthermore, that the majority of human AMs after transplant are derived from circulating monocytes.
中文翻译:
健康衰老期间和移植后人类单核细胞和气道巨噬细胞的动态
人类肺部气道巨噬细胞(AM)的个体发育及其对疾病的贡献尚不清楚。在小鼠中,衰老与外周 AM 比例的增加有关,而不是围产期衍生的 AM。我们试图了解人肺在健康衰老过程中和移植后的 AM 个体发育。我们对健康志愿者在婴儿/儿童期(2-12 岁)、成熟期(20-50 岁)和老年期(> 50 岁)的外周血和气道中的单核细胞/巨噬细胞群进行了特征分析。对从性别不匹配的肺移植受者中分离出的气道炎症细胞进行了单细胞 RNA 测序 (scRNA-seq)。在健康老龄化过程中,血液和支气管肺泡灌洗液 (BAL) 经典单核细胞的比例在成年期达到峰值,并在老年人中下降。来自肺移植受者的 BAL 细胞的 scRNA-seq 表明,移植后,大多数 AM 源自受者。这些数据表明,在衰老过程中,外周单核细胞表型与气道中发现的表型一致,此外,移植后大多数人类 AM 来源于循环单核细胞。
更新日期:2020-01-09
中文翻译:
健康衰老期间和移植后人类单核细胞和气道巨噬细胞的动态
人类肺部气道巨噬细胞(AM)的个体发育及其对疾病的贡献尚不清楚。在小鼠中,衰老与外周 AM 比例的增加有关,而不是围产期衍生的 AM。我们试图了解人肺在健康衰老过程中和移植后的 AM 个体发育。我们对健康志愿者在婴儿/儿童期(2-12 岁)、成熟期(20-50 岁)和老年期(> 50 岁)的外周血和气道中的单核细胞/巨噬细胞群进行了特征分析。对从性别不匹配的肺移植受者中分离出的气道炎症细胞进行了单细胞 RNA 测序 (scRNA-seq)。在健康老龄化过程中,血液和支气管肺泡灌洗液 (BAL) 经典单核细胞的比例在成年期达到峰值,并在老年人中下降。来自肺移植受者的 BAL 细胞的 scRNA-seq 表明,移植后,大多数 AM 源自受者。这些数据表明,在衰老过程中,外周单核细胞表型与气道中发现的表型一致,此外,移植后大多数人类 AM 来源于循环单核细胞。