当前位置: X-MOL 学术Macromol. Biosci. › 论文详情
Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)
Temperature-Sensitive Amphiphilic Non-Ionic Triblock Copolymers for Enhanced In Vivo Skeletal Muscle Transfection.
Macromolecular Bioscience ( IF 4.6 ) Pub Date : 2020-01-09 , DOI: 10.1002/mabi.201900276
Bazoly Rasolonjatovo 1, 2 , Nicolas Illy 3 , Véronique Bennevault 3, 4 , Jérôme Mathé 1, 2 , Patrick Midoux 5 , Tony Le Gall 6 , Thomas Haudebourg 7 , Tristan Montier 6 , Pierre Lehn 6 , Bruno Pitard 7 , Herve Cheradame 1, 2 , Cécile Huin 3, 4 , Philippe Guégan 3
Affiliation  

It is reported that low concentration of amphiphilic triblock copolymers of pMeOx‐b‐pTHF‐b‐pMeOx structure (TBCPs) improves gene expression in skeletal muscle upon intramuscular co‐injection with plasmid DNA. Physicochemical studies carried out to understand the involved mechanism show that a phase transition of TBCPs under their unimer state is induced when the temperature is elevated from 25 to 37 °C, the body temperature. Several lines of evidences suggest that TBCP insertion in a lipid bilayer causes enough lipid bilayer destabilization and even pore formation, a phenomenon heightened during the phase transition of TBCPs. Interestingly, this property allows DNA translocation across the lipid bilayer model. Overall, the results indicate that TBCPs exhibiting a phase transition at the body temperature is promising to favor in vivo pDNA translocation in skeletal muscle cells for gene therapy applications.

中文翻译:

温度敏感的两亲非离子三嵌段共聚物,可增强体内骨骼肌的转染。

据报道pMeOx‐ b‐ pTHF‐ b的两亲三嵌段共聚物浓度低‐pMeOx结构(TBCP)可在与质粒DNA肌肉内注射后改善骨骼肌中的基因表达。为了解所涉及的机理而进行的理化研究表明,当温度从25升高到37°C(人体温度)时,TBCP在其单体状态下会发生相变。几条证据表明,将TBCP插入脂质双分子层会引起足够的脂质双分子层不稳定,甚至形成孔,这是在TBCP的相变过程中加剧的现象。有趣的是,这种特性使DNA跨脂质双层模型易位。总体而言,结果表明,在体温下表现出相变的TBCP有望促进基因疗法在骨骼肌细胞中的体内pDNA易位。
更新日期:2020-01-09
down
wechat
bug