Acinetobacter baumannii and Pseudomonas aeruginosa are the most relevant Gram-negative bacteria associated with hospital and opportunistic infections. This study aimed to evaluate the dynamics of drug-resistant A. baumannii and P. aeruginosa and biofilm formers from two public hospitals in northeastern Brazil. One hundred isolates (35 from A. baumannii and 65 from P. aeruginosa) were identified using the automated Vitek^®2 Compact method (bioMérieux) and confirmed using the MALDI-TOF (MS) mass spectrometry technique. Molecular experiments were performed by polymerase chain reaction (PCR) to detect the frequency of bla_KPC, bla_IMP, bla_VIM, and bla_SHV genes. The biofilm formation potential was evaluated using crystal violet in Luria Bertani Miller and trypticase soy broth culture media under the following conditions: at standard concentration, one quarter (25%) of the standard concentration and supplemented with 1% glucose. In addition, the genetic diversity of the isolates was verified by the ERIC-PCR technique. Isolates presented distinct resistance profiles with a high level of beta-lactam resistance. The highest index of genes detected was bla_KPC (60%), followed by bla_SHV (39%), bla_VIM (8%), and bla_IMP (1%). All the isolates were sensitive to the polymyxins tested and formed biofilms at different intensities. Twelve clones of A. baumannii and eight of P. aeruginosa were identified, of which few were indicative of intra- and interhospital dissemination. This study reveals the dispersion dynamics of these isolates in the hospital environment. The results demonstrate the importance of monitoring programs to combat the spread of these pathogens.

中文翻译：

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院内和院内耐药鲍曼不动杆菌和铜绿假单胞菌的发生和多样性。

鲍曼不动杆菌和铜绿假单胞菌是与医院和机会感染相关的最相关的革兰氏阴性细菌。这项研究旨在评估耐药A的动力学。鲍曼不动杆菌和P。来自巴西东北部两家公立医院的铜绿和生物膜形成物。一百分离株（35从鲍曼不动杆菌和65从绿脓杆菌）使用自动化维特克识别^® 2 Compact方法（生物梅里埃），并使用MALDI-TOF（MS）质谱技术证实。通过聚合酶链反应（PCR）进行了分子实验，以检测bla _KPC，bla _IMP，bla _VIM和bla _SHV基因。在以下条件下，使用Luria Bertani Miller中的结晶紫和胰酶解大豆肉汤培养基，评估生物膜形成的潜力：标准浓度，标准浓度的四分之一（25％）并补充1％葡萄糖。另外，通过ERIC-PCR技术验证了分离物的遗传多样性。分离物呈现出不同的抗性，并具有高水平的β-内酰胺抗性。检测到的最高基因索引是bla _KPC（60％），其次是bla _SHV（39％），bla _VIM（8％）和bla _IMP（1％）。所有分离株均对测试的多粘菌素敏感，并在不同强度下形成生物膜。A的12个克隆。鲍曼尼和P的八个。确定了铜绿假单胞菌，其中很少有迹象表明院内和院际散布。这项研究揭示了这些分离株在医院环境中的分散动力学。结果表明，监测计划必须打击这些病原体的传播。